Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-7-16
pubmed:abstractText
The effects of chylomicron remnants on lipid accumulation in J774 macrophages, and the incorporation of radioactivity from remnant lipids radiolabelled with [3H]oleate into cellular lipids was investigated. After 24 h of incubation with chylomicron remnants, there was considerable accumulation of lipid within the cells as assessed by staining with oil red O, indicating that the particles induce the formation of foam cells. Fatty acids released from the radiolabelled remnant lipids after uptake were found to be incorporated into cellular triacylglycerol (52%), phospholipid (37%) and cholesteryl ester (8%), but at higher remnant concentrations, the proportion used for triacylglycerol formation increased (up to 73%). When the macrophages were shifted into a pro-oxidising or pro-reducing state by incubation (24 h) with CuSO4 (2.5 microM) or N-acetylcysteine (5 mM), respectively, the incorporation of [3H]oleate from remnant lipid into cellular triacylglycerol and phospholipid was increased by 20-30% in the more oxidised as compared with the more reduced cells. These findings indicate that exposure of J774 macrophages to chylomicron remnants leads to the accumulation of lipid within the cells, and that this process is enhanced by pro-oxidising conditions. We conclude, therefore, that both lipids of dietary origin and the redox balance within macrophages may have a significant role in the induction of foam cell formation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1018-1172
pubmed:author
pubmed:copyrightInfo
Copyright 2001 S. Karger AG, Basel
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
350-60
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11455206-Acetylcysteine, pubmed-meshheading:11455206-Animals, pubmed-meshheading:11455206-Cell Line, pubmed-meshheading:11455206-Cholesterol, pubmed-meshheading:11455206-Cholesterol Esters, pubmed-meshheading:11455206-Chylomicrons, pubmed-meshheading:11455206-Copper Sulfate, pubmed-meshheading:11455206-Dietary Fats, pubmed-meshheading:11455206-Fatty Acids, Nonesterified, pubmed-meshheading:11455206-Foam Cells, pubmed-meshheading:11455206-Lipid Metabolism, pubmed-meshheading:11455206-Lipids, pubmed-meshheading:11455206-Macrophages, pubmed-meshheading:11455206-Male, pubmed-meshheading:11455206-Mice, pubmed-meshheading:11455206-Oleic Acid, pubmed-meshheading:11455206-Oxidation-Reduction, pubmed-meshheading:11455206-Phospholipids, pubmed-meshheading:11455206-Rats, pubmed-meshheading:11455206-Rats, Wistar, pubmed-meshheading:11455206-Triglycerides, pubmed-meshheading:11455206-Tritium
pubmed:articleTitle
The internal redox balance of the cells influences the metabolism of lipids of dietary origin by J774 macrophages: implications for foam cell formation.
pubmed:affiliation
Istituto Superiore di Sanità, Laboratory of Metabolism and Pathological Biochemistry, Rome, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't