Source:http://linkedlifedata.com/resource/pubmed/id/11455203
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2001-7-16
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| pubmed:abstractText |
Ca2+ inflow via store-operated Ca2+ channels was investigated in rings of rat tail and basilar arteries kept in serum-free organ culture, which is known to preserve the contractility of the vascular smooth muscle. After culture for 3-4 days, Ca2+ release from intracellular stores in response to caffeine (20 mM) was augmented 2- to 4-fold. Following depletion of intracellular Ca2+ stores by caffeine and thapsigargin (10 microM), addition of Ca2+ (2.5 mM) caused an increase in the intracellular Ca2+ concentration which was 2-3 times greater in cultured than in freshly dissected rings, and was not affected by verapamil (10 microM). In contrast, L-type Ca2+ channel currents were decreased by 20% after culture. While freshly dissected rings developed no or very little force in response to the addition of Ca2+ after store depletion, cultured rings developed 42% (tail artery) and 60% (basilar artery) of the force of high-K+-induced contractions. These contractions in cultured vessels were insensitive to verapamil but could be completely relaxed by SKF-96365 (30 microM). Store depletion by caffeine increased the Mn2+ quench rate 3- to 4-fold in freshly dissected as well as cultured tail artery, while there was no increase in freshly dissected basilar artery, but a 3-fold increase in cultured basilar artery. Uptake of Ca2+ into intracellular stores was twice as rapid in cultured as in freshly dissected tail artery. This study shows that organ culture of vascular smooth muscle tissue causes changes in Ca2+ handling, resembling the pattern seen in dedifferentiating smooth muscle cells in culture, although contractile properties are maintained.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(2-(3-(4-methoxyphenyl)propoxy)-4-...,
http://linkedlifedata.com/resource/pubmed/chemical/Caffeine,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels, L-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Manganese,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin,
http://linkedlifedata.com/resource/pubmed/chemical/Verapamil
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| pubmed:status |
MEDLINE
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| pubmed:issn |
1018-1172
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2001 S. Karger AG, Basel
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| pubmed:issnType |
Print
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| pubmed:volume |
38
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
324-31
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11455203-Animals,
pubmed-meshheading:11455203-Basilar Artery,
pubmed-meshheading:11455203-Caffeine,
pubmed-meshheading:11455203-Calcium,
pubmed-meshheading:11455203-Calcium Channel Blockers,
pubmed-meshheading:11455203-Calcium Channels,
pubmed-meshheading:11455203-Calcium Channels, L-Type,
pubmed-meshheading:11455203-Culture Media, Serum-Free,
pubmed-meshheading:11455203-Electric Conductivity,
pubmed-meshheading:11455203-Female,
pubmed-meshheading:11455203-Imidazoles,
pubmed-meshheading:11455203-Manganese,
pubmed-meshheading:11455203-Muscle, Smooth, Vascular,
pubmed-meshheading:11455203-Muscle Contraction,
pubmed-meshheading:11455203-Organ Culture Techniques,
pubmed-meshheading:11455203-Potassium,
pubmed-meshheading:11455203-Rats,
pubmed-meshheading:11455203-Rats, Sprague-Dawley,
pubmed-meshheading:11455203-Tail,
pubmed-meshheading:11455203-Thapsigargin,
pubmed-meshheading:11455203-Verapamil
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| pubmed:articleTitle |
Increased store-operated Ca2+ entry into contractile vascular smooth muscle following organ culture.
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| pubmed:affiliation |
Department of Physiological Sciences, Lund University, Lund, Sweden.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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