Linked Life Data

11454959

Source:http://linkedlifedata.com/resource/pubmed/id/11454959

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt. 2
pubmed:dateCreated
2001-7-16
pubmed:abstractText
1. Ca(2+)-activated chloride currents (I(Cl(Ca))) were recorded from smooth muscle cells isolated from rabbit pulmonary (PA) and coronary artery (CA) as well as rabbit portal vein (PV). The characteristics and regulation by Ca(2+)-calmodulin-dependent kinase II (CaMKII) were compared between the three cell types. 2. In PA and CA myocytes dialysed and superfused with K+ -free media, pipette solutions containing fixed levels of free Ca(2+) in the range of 250 nM to 1 microM evoked well sustained, outwardly rectifying I(Cl(Ca)) currents in about 90 % of cells. The CaMKII inhibitor KN-93 (5 microM) increased the amplitude of I(Cl(Ca)) in PA and CA myocytes. However, the threshold intracellular Ca(2+) concentration for detecting this effect was different in the two arterial cell types. KN-93 also enhanced the rate of activation of the time-dependent current during depolarising steps, slowed the kinetics of the tail current following repolarisation, and induced a negative shift of the steady-state activation curve. 3. In PA myocytes, the effects of KN-93 were not mirrored by its inactive analogue KN-92 but were reproduced by the inclusion of autocamtide-2-related CaMKII inhibitory peptide (ARIP) in the pipette solution. Cell dialysis with constitutively active CaMKII (30 nM) significantly reduced I(Cl(Ca)) evoked by 500 nM Ca(2+). 4. In PV myocytes, I(Cl(Ca)) was evoked by pipette solutions containing up to 1 microM free Ca(2+) in less than 40 % of cells. Application of KN-93 to cells where I(Cl(Ca)) was sustained produced a small inhibition (approximately 25%) of the current in 70 % of the cells. 5. The present study shows that regulation of Ca(2+)-dependent Cl(-) channels by CaMKII differs between arterial and portal vein myocytes.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-10226150, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-10454491, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-10564126, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-10613919, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-10712242, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-10912466, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-1315424, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-1403823, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-1662507, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-1696988, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-1849722, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-2473199, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-2600861, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-5653881, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-7479960, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-7626114, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-7771540, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-7923619, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-8229796, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-8271266, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-8576214, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-8680728, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-8769982, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-8817383, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-9133364, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-9176262, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-9232352, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-9405714, http://linkedlifedata.com/resource/pubmed/commentcorrection/11454959-9458724
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-3751
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
534
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
395-408
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:11454959-Animals, pubmed-meshheading:11454959-Benzylamines, pubmed-meshheading:11454959-Calcium, pubmed-meshheading:11454959-Calcium-Calmodulin-Dependent Protein Kinase Type 2, pubmed-meshheading:11454959-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:11454959-Chloride Channels, pubmed-meshheading:11454959-Chlorides, pubmed-meshheading:11454959-Enzyme Inhibitors, pubmed-meshheading:11454959-Membrane Potentials, pubmed-meshheading:11454959-Muscle, Smooth, Vascular, pubmed-meshheading:11454959-Muscle Fibers, Skeletal, pubmed-meshheading:11454959-Patch-Clamp Techniques, pubmed-meshheading:11454959-Peptides, pubmed-meshheading:11454959-Phosphorylation, pubmed-meshheading:11454959-Portal Vein, pubmed-meshheading:11454959-Pulmonary Artery, pubmed-meshheading:11454959-Rabbits, pubmed-meshheading:11454959-Sulfonamides
pubmed:year
2001
pubmed:articleTitle
Differential regulation of Ca(2+)-activated Cl(-) currents in rabbit arterial and portal vein smooth muscle cells by Ca(2+)-calmodulin-dependent kinase.
pubmed:affiliation
Department of Pharmacology and Clinical Pharmacology, St George's Hospital Medical School, London, UK. i.greenwood@sghms.ac.uk
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't