Linked Life Data

11454415

Source:http://linkedlifedata.com/resource/pubmed/id/11454415

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-7-16
pubmed:abstractText
We studied the spectrins in developing hippocampal tissue in vivo and in vitro to learn how they contribute to the organization of synaptic and extrasynaptic regions of the neuronal plasma membrane. beta-Spectrin, but not beta-fodrin or alpha-fodrin, increased substantially during postnatal development in the hippocampus, where it was localized in neurons but not in astrocytes. Immunoprecipitations from neonatal and adult hippocampal extracts suggest that while both beta-spectrin and beta-fodrin form heteromers with alpha-fodrin, oligomers containing all three subunits are also present. At the subcellular level, beta-fodrin and alpha-fodrin were present in the cell bodies, dendrites, and axons of pyramidal-like neurons in culture, as well as in astrocytes. beta-Spectrin, by contrast, was absent from axons but present in cell bodies and dendrites, where it was organized in a loose, membrane-associated meshwork that lacked alpha-fodrin. A similar meshwork was also apparent in pyramidal neurons in vivo. At some dendritic spines, alpha-fodrin was present in the necks but not in the heads, whereas beta-spectrin was present at significant levels in the spine heads. The presence of significant amounts of beta-spectrin without an accompanying alpha-fodrin subunit was confirmed by immunoprecipitations from extracts of adult hippocampus. Our results suggest that the spectrins in hippocampal neurons can assemble to form different membrane-associated structures in distinct membrane domains, including those at synapses.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0165-3806
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
129
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
81-93
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Spectrins in developing rat hippocampal cells.
pubmed:affiliation
Department of Physiology, University of Maryland School of Medicine, 655 W. Baltimore Street, Baltimore, MD 21201, USA. jursitti@umaryland.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.