Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-7-16
pubmed:abstractText
The glucagon receptor mediates the actions of glucagon on carbohydrate metabolism by the liver and on insulin release by the pancreatic beta-cell, which are key processes in the control of glucose homeostasis. The 5'-region of the mouse glucagon receptor gene has been recently cloned and two functional promoters were characterized. In the present study, we show that most of the glucagon receptor mRNA was transcribed from the distal promoter, in the mouse liver. In the distal promoter region, a GC-rich sequence with five putative binding sites for the Sp family of transcription factors was localized. To elucidate the role of these Sp1-binding sites in the mouse MIN6 beta-cell line, the expression of reporter gene constructs containing deletion or point mutation of each site was carried out. Selective mutation of the second Sp1-binding site decreased the activity of the distal promoter. Electrophoretic mobility shift assay with a DNA fragment spanning the three first Sp1 sites confirmed that the second site bound specifically MIN6 nuclear proteins, and supershift using specific Sp antibodies demonstrated that it interacted with Sp3 but not Sp1 transcription factors. These data illustrate that the basal expression of the mouse glucagon receptor gene, driven by the distal promoter, requires an Sp1-binding site that binds Sp3 proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Academic Press.
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
285
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
838-44
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11453669-5' Untranslated Regions, pubmed-meshheading:11453669-Animals, pubmed-meshheading:11453669-Binding Sites, pubmed-meshheading:11453669-Blotting, Western, pubmed-meshheading:11453669-Cells, Cultured, pubmed-meshheading:11453669-DNA-Binding Proteins, pubmed-meshheading:11453669-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:11453669-Gene Expression, pubmed-meshheading:11453669-Genes, Reporter, pubmed-meshheading:11453669-Islets of Langerhans, pubmed-meshheading:11453669-Liver, pubmed-meshheading:11453669-Mice, pubmed-meshheading:11453669-Mice, Inbred C57BL, pubmed-meshheading:11453669-Mutagenesis, Site-Directed, pubmed-meshheading:11453669-Nuclear Proteins, pubmed-meshheading:11453669-Promoter Regions, Genetic, pubmed-meshheading:11453669-RNA, Messenger, pubmed-meshheading:11453669-Receptors, Glucagon, pubmed-meshheading:11453669-Sp1 Transcription Factor, pubmed-meshheading:11453669-Sp3 Transcription Factor, pubmed-meshheading:11453669-Transcription, Genetic, pubmed-meshheading:11453669-Transcription Factors
pubmed:year
2001
pubmed:articleTitle
Role of the Sp family of transcription factors on glucagon receptor gene expression.
pubmed:affiliation
INSERM U376, CHU Arnaud de Villeneuve, 371, rue Doyen G. Giraud, 34295 Montpellier Cedex 5, France.
pubmed:publicationType
Journal Article