Linked Life Data

11453631

Source:http://linkedlifedata.com/resource/pubmed/id/11453631

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-7-16
pubmed:abstractText
Memory T cells in inflamed skin express the cutaneous lymphocyte-associated antigen (CLA), a glycosylated epitope defined by the mAb HECA-452. We previously reported that on T cells, CLA occurs almost exclusively on the protein backbone of P-selectin glycoprotein ligand-1 (PSGL-1). T cells exhibiting the CLA isoform of PSGL-1 can tether and roll on both E- and P-selectin, while T cells expressing PSGL-1 without the CLA epitope do not bind E-selectin, though they may bind P-selectin. We show here that circulating neutrophils and monocytes, and cultured blood dendritic cells, also express CLA almost entirely as an isoform of PSGL-1. These cells all tether and roll on both E- and P-selectin. A chimeric fusion protein incorporating the 19 N-terminal amino acids of mature PSGL-1 exhibited HECA-452 immunoreactivity and supported rolling of CHO cells expressing either E- or P-selectin. These findings indicate a site for the CLA modification within the distal tip of PSGL-1, previously shown to be critical for P-selectin binding and to mediate some, but not all, of the E-selectin binding of PSGL-1. We hypothesize that the types of circulating leukocytes discussed above all use CLA/PSGL-1 to tether and roll on E- and P-selectin along the vascular endothelium.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Academic Press.
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
285
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
577-87
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:11453631-Animals, pubmed-meshheading:11453631-Antibodies, Monoclonal, pubmed-meshheading:11453631-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:11453631-Antigens, Neoplasm, pubmed-meshheading:11453631-CHO Cells, pubmed-meshheading:11453631-Cell Separation, pubmed-meshheading:11453631-Cells, Cultured, pubmed-meshheading:11453631-Cricetinae, pubmed-meshheading:11453631-Dendritic Cells, pubmed-meshheading:11453631-E-Selectin, pubmed-meshheading:11453631-Humans, pubmed-meshheading:11453631-Inflammation, pubmed-meshheading:11453631-Membrane Glycoproteins, pubmed-meshheading:11453631-Monocytes, pubmed-meshheading:11453631-Neutrophils, pubmed-meshheading:11453631-P-Selectin, pubmed-meshheading:11453631-Peptide Fragments, pubmed-meshheading:11453631-Protein Binding, pubmed-meshheading:11453631-Protein Isoforms, pubmed-meshheading:11453631-Recombinant Fusion Proteins, pubmed-meshheading:11453631-Skin, pubmed-meshheading:11453631-Stress, Mechanical, pubmed-meshheading:11453631-T-Lymphocytes
pubmed:year
2001
pubmed:articleTitle
Neutrophils, monocytes, and dendritic cells express the same specialized form of PSGL-1 as do skin-homing memory T cells: cutaneous lymphocyte antigen.
pubmed:affiliation
Harvard Skin Disease Research Center, Dept. of Dermatology, Brigham and Women's Hospital, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.