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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5531
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pubmed:dateCreated |
2001-8-3
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pubmed:abstractText |
The 21-nucleotide small temporal RNA (stRNA) let-7 regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
293
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
834-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11452083-Animals,
pubmed-meshheading:11452083-Blotting, Northern,
pubmed-meshheading:11452083-Drosophila melanogaster,
pubmed-meshheading:11452083-Endoribonucleases,
pubmed-meshheading:11452083-Gene Expression Regulation, Developmental,
pubmed-meshheading:11452083-HeLa Cells,
pubmed-meshheading:11452083-Humans,
pubmed-meshheading:11452083-Nucleic Acid Conformation,
pubmed-meshheading:11452083-Protein Structure, Tertiary,
pubmed-meshheading:11452083-RNA, Double-Stranded,
pubmed-meshheading:11452083-RNA, Helminth,
pubmed-meshheading:11452083-RNA, Messenger,
pubmed-meshheading:11452083-RNA Precursors,
pubmed-meshheading:11452083-RNA Processing, Post-Transcriptional,
pubmed-meshheading:11452083-Ribonuclease III,
pubmed-meshheading:11452083-Transcription, Genetic,
pubmed-meshheading:11452083-Transfection
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pubmed:year |
2001
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pubmed:articleTitle |
A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA.
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pubmed:affiliation |
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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