Linked Life Data

11446372

Source:http://linkedlifedata.com/resource/pubmed/id/11446372

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-7-10
pubmed:databankReference
pubmed:abstractText
Phospholipase D (PLD, EC 3.1.4.4.) has been implicated in a variety of plant processes, including signalling. In Arabidopsis thaliana a PLD gene family has been described and individual members classified into alpha-, beta- and gamma-classes. Here we describe a second PLD gene family in tomato (Lycopersicon esculentum) that includes three alpha- and two beta-classes. Different expression patterns in plant organs were observed for each PLD. In testing a variety of stress treatments on tomato cell suspensions, PLDbeta1 mRNA was found to rapidly and specifically accumulate in response to the fungal elicitor xylanase. The greatest increase was found 2 h after treatment with 100 microg m1(-1) xylanase (ninefold). In vivo PLD activity increased nearly threefold over a 1.5 h period of treatment. When the elicitor was injected into tomato leaves, PLDbeta1 mRNA accumulation peaked at 2 h (threefold increase), before decreasing to background levels within 72 h. Mutant, non-active xylanase was as effective as the active enzyme in eliciting a response, suggesting that xylanase itself, and not the products resulting from its activity, functioned as an elicitor. When chitotetraose was used as elicitor, no PLDbeta1 mRNA accumulation was observed, thus it is not a general response to elicitation. Together these data show that PLD genes are differentially regulated, reflecting potential differences in cellular function. The possibility that PLDbeta1 is a signalling enzyme is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0960-7412
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
237-47
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11446372-Amino Acid Sequence, pubmed-meshheading:11446372-Cells, Cultured, pubmed-meshheading:11446372-Cloning, Molecular, pubmed-meshheading:11446372-Cold Temperature, pubmed-meshheading:11446372-DNA, Complementary, pubmed-meshheading:11446372-DNA, Plant, pubmed-meshheading:11446372-Gene Expression Regulation, Enzymologic, pubmed-meshheading:11446372-Gene Expression Regulation, Plant, pubmed-meshheading:11446372-Lycopersicon esculentum, pubmed-meshheading:11446372-Molecular Sequence Data, pubmed-meshheading:11446372-Multigene Family, pubmed-meshheading:11446372-Osmotic Pressure, pubmed-meshheading:11446372-Phospholipase D, pubmed-meshheading:11446372-Plant Leaves, pubmed-meshheading:11446372-RNA, Messenger, pubmed-meshheading:11446372-Sequence Alignment, pubmed-meshheading:11446372-Xylan Endo-1,3-beta-Xylosidase, pubmed-meshheading:11446372-Xylosidases
pubmed:year
2001
pubmed:articleTitle
Characterization of five tomato phospholipase D cDNAs: rapid and specific expression of LePLDbeta1 on elicitation with xylanase.
pubmed:affiliation
Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands. laxalt@science.uva.nl
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't