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pubmed-article:11445278pubmed:abstractTextCardiac arrest and resuscitation were used to induce brain damage and susceptibility to sound-triggered seizures in Sprague-Dawley rats. Glucose preloading was used to vary seizure susceptibility. Because loop diuretics can block these seizures, we investigated changes in KCC2, a potassium-chloride cotransporter, in the inferior colliculus - the origin of the seizures. Using polymerase chain reaction (PCR), we found that collicular KCC2 mRNA levels covaried with seizure susceptibility in these animals. Using quantitative PCR, we found that a fivefold increase in collicular KCC2 mRNA levels was associated with a doubling of seizure incidence. A hypothesis linking KCC2 activity to seizure susceptibility is presented.lld:pubmed
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pubmed-article:11445278pubmed:articleTitleThe mRNA level of the potassium-chloride cotransporter KCC2 covaries with seizure susceptibility in inferior colliculus of the post-ischemic audiogenic seizure-prone rat.lld:pubmed
pubmed-article:11445278pubmed:affiliationDepartment of Anatomical Sciences and Neurobiology, School of Medicine, University of Louisville, Louisville, KY, USA. khreid01@louisville.edulld:pubmed
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