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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2001-7-10
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pubmed:abstractText |
Cardiac arrest and resuscitation were used to induce brain damage and susceptibility to sound-triggered seizures in Sprague-Dawley rats. Glucose preloading was used to vary seizure susceptibility. Because loop diuretics can block these seizures, we investigated changes in KCC2, a potassium-chloride cotransporter, in the inferior colliculus - the origin of the seizures. Using polymerase chain reaction (PCR), we found that collicular KCC2 mRNA levels covaried with seizure susceptibility in these animals. Using quantitative PCR, we found that a fivefold increase in collicular KCC2 mRNA levels was associated with a doubling of seizure incidence. A hypothesis linking KCC2 activity to seizure susceptibility is presented.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0304-3940
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
308
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
29-32
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11445278-Animals,
pubmed-meshheading:11445278-Blood Glucose,
pubmed-meshheading:11445278-Brain Ischemia,
pubmed-meshheading:11445278-Carrier Proteins,
pubmed-meshheading:11445278-Chloride Channels,
pubmed-meshheading:11445278-Disease Models, Animal,
pubmed-meshheading:11445278-Disease Susceptibility,
pubmed-meshheading:11445278-Diuretics,
pubmed-meshheading:11445278-Epilepsy,
pubmed-meshheading:11445278-Epilepsy, Reflex,
pubmed-meshheading:11445278-Genetic Predisposition to Disease,
pubmed-meshheading:11445278-Glucose,
pubmed-meshheading:11445278-Heart Arrest, Induced,
pubmed-meshheading:11445278-Inferior Colliculi,
pubmed-meshheading:11445278-Neurons,
pubmed-meshheading:11445278-Polymerase Chain Reaction,
pubmed-meshheading:11445278-RNA, Messenger,
pubmed-meshheading:11445278-Rats,
pubmed-meshheading:11445278-Rats, Sprague-Dawley,
pubmed-meshheading:11445278-Symporters,
pubmed-meshheading:11445278-Up-Regulation
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pubmed:year |
2001
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pubmed:articleTitle |
The mRNA level of the potassium-chloride cotransporter KCC2 covaries with seizure susceptibility in inferior colliculus of the post-ischemic audiogenic seizure-prone rat.
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pubmed:affiliation |
Department of Anatomical Sciences and Neurobiology, School of Medicine, University of Louisville, Louisville, KY, USA. khreid01@louisville.edu
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pubmed:publicationType |
Journal Article
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