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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0018270,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0377261,
umls-concept:C0525037,
umls-concept:C0809183,
umls-concept:C1314939,
umls-concept:C1705280,
umls-concept:C1705748,
umls-concept:C2717940
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pubmed:issue |
2
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pubmed:dateCreated |
2001-7-10
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pubmed:abstractText |
Saikosaponin a, a purified ingredient of Chinese herb with known antitumor activity, can inhibit cell growth and DNA synthesis of hepatoma cell line HepG2. Both mRNA and protein of the CDK inhibitor p-16(INK4a) and p-15(INK4b) in HepG2 were greatly induced by saikosaponin a while that of p-21(CIP), p-27(KIP) and other cell cycle related genes were not. In addition, reduced phosphorylation of RB protein is observed in saikosaponin a-treated HepG2. Staurosporin, one of the PKC inhibitors, significantly prevented the saikosaponin a induced growth inhibition suggesting PKC pathway be involved. On the other hand, the phorbol ester tumor promoter TPA (12-O-Tetredecanolyphorbol 13-acetate) also inhibited HepG2 growth and specifically induced p-16(INK4a) and p-15(INK4b) mRNA expression. The results suggest that both saikosaponin a and TPA-induced HepG2 growth inhibition are associated with p-15(INK4a) and p-16(INK4b) gene expression and might be mediated by PKC signaling pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents, Phytogenic,
http://linkedlifedata.com/resource/pubmed/chemical/CDKN2B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Oleanolic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sapogenins,
http://linkedlifedata.com/resource/pubmed/chemical/Saponins,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/saikosaponin
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
285
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
183-7
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11444823-Antineoplastic Agents, Phytogenic,
pubmed-meshheading:11444823-Carcinoma, Hepatocellular,
pubmed-meshheading:11444823-Carrier Proteins,
pubmed-meshheading:11444823-Cell Cycle Proteins,
pubmed-meshheading:11444823-Cell Division,
pubmed-meshheading:11444823-Cyclin-Dependent Kinase Inhibitor p15,
pubmed-meshheading:11444823-Cyclin-Dependent Kinase Inhibitor p16,
pubmed-meshheading:11444823-Enzyme Inhibitors,
pubmed-meshheading:11444823-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:11444823-Genes, Tumor Suppressor,
pubmed-meshheading:11444823-Humans,
pubmed-meshheading:11444823-Liver Neoplasms,
pubmed-meshheading:11444823-Oleanolic Acid,
pubmed-meshheading:11444823-Protein Kinase C,
pubmed-meshheading:11444823-RNA, Messenger,
pubmed-meshheading:11444823-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11444823-Sapogenins,
pubmed-meshheading:11444823-Saponins,
pubmed-meshheading:11444823-Signal Transduction,
pubmed-meshheading:11444823-Tetradecanoylphorbol Acetate,
pubmed-meshheading:11444823-Transcription, Genetic,
pubmed-meshheading:11444823-Tumor Cells, Cultured,
pubmed-meshheading:11444823-Tumor Suppressor Proteins
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pubmed:year |
2001
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pubmed:articleTitle |
Involvement of p-15(INK4b) and p-16(INK4a) gene expression in saikosaponin a and TPA-induced growth inhibition of HepG2 cells.
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pubmed:affiliation |
Department of Medical Technology, Tzu Chi University, No. 701, Chung Yang Rd., Sec. 3, Hualien, Taiwan 970. wuws@mail.tcu.edu.tw
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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