Linked Life Data

11441112

Source:http://linkedlifedata.com/resource/pubmed/id/11441112

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-7-6
pubmed:abstractText
Leukocyte recruitment into the infected peritoneal cavity consists of an early, predominant polymorphonuclear leukocyte (PMN) influx and subsequent, prolonged mononuclear cell migration phase. Although chemokine secretion by resident peritoneal cells plays a primary role in mediating this migration, the mechanisms involved in controlling the switch in phenotype of cell infiltrate remain unclear. The present study investigates a potential role for the Th1-type cytokine IFN-gamma in the process of leukocyte recruitment into the peritoneal cavity. Stimulation of cultured human peritoneal mesothelial cells with IFN-gamma (1-100 U/ml) alone or in combination with IL-1beta (100 pg/ml) or TNF-alpha (1000 pg/ml) resulted in significant up-regulation of monocyte chemoattractant protein-1 and RANTES protein secretion. In contrast, IFN-gamma inhibited basal and IL-1beta-, and TNF-alpha-induced production of IL-8. The modulating effects of IFN-gamma on chemokine production occurred at the level of gene expression, and the degree of regulation observed was dependent on the doses of IL-1beta and TNF-alpha used. Analysis of the functional effects of IFN-gamma on IL-1beta-induced transmesothelial PMN migration with an in vitro human transmigration system and an in vivo murine model of peritoneal inflammation demonstrated that IFN-gamma was able to down-regulate PMN migration induced by optimal doses of IL-1beta. These effects were mediated in vivo via down-regulation of CXC chemokine synthesis. These findings suggest that IFN-gamma may play a role in controlling the phenotype of infiltrating leukocyte during the course of an inflammatory response, in part via regulation of resident cell chemokine synthesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
167
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1028-38
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11441112-Adjuvants, Immunologic, pubmed-meshheading:11441112-Animals, pubmed-meshheading:11441112-Cells, Cultured, pubmed-meshheading:11441112-Chemokine CCL2, pubmed-meshheading:11441112-Chemokine CCL5, pubmed-meshheading:11441112-Chemokines, pubmed-meshheading:11441112-Chemotaxis, Leukocyte, pubmed-meshheading:11441112-Diffusion Chambers, Culture, pubmed-meshheading:11441112-Dose-Response Relationship, Immunologic, pubmed-meshheading:11441112-Drug Synergism, pubmed-meshheading:11441112-Epithelial Cells, pubmed-meshheading:11441112-Humans, pubmed-meshheading:11441112-Interferon-gamma, pubmed-meshheading:11441112-Interleukin-1, pubmed-meshheading:11441112-Interleukin-8, pubmed-meshheading:11441112-Mice, pubmed-meshheading:11441112-Mice, Inbred BALB C, pubmed-meshheading:11441112-Neutrophil Infiltration, pubmed-meshheading:11441112-Peritoneal Cavity, pubmed-meshheading:11441112-RNA, Messenger, pubmed-meshheading:11441112-Time Factors
pubmed:year
2001
pubmed:articleTitle
Differential regulation of chemokine production in human peritoneal mesothelial cells: IFN-gamma controls neutrophil migration across the mesothelium in vitro and in vivo.
pubmed:affiliation
Institute of Nephrology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, Wales, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't