Source:http://linkedlifedata.com/resource/pubmed/id/11440191
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2001-7-6
|
| pubmed:abstractText |
Human visceral leishmaniosis is endemic in Southern Italy, where the dog is the main reservoir of viscerotropic strains of Leishmania infantum. The release of nitric oxide (NO) by interferon (IFN)-gamma-activated macrophages is an important leishmanicidal mechanism in several animal species. In this work NO production, phagocytosis and killing capacity of monocyte-derived dog macrophages were evaluated in vitro before and after administration of a vaccine composed of killed Leishmania infantum promastigotes. Moreover, IFN-gamma content was measured in concanavalin A-activated dog peripheral blood mononuclear cell (PBMC) supernatants employed for macrophage stimulation. Phagocytosis, killing capacity and NO production by canine macrophages increased significantly 1 month after vaccine administration, and the increase also persisted 5 months later. In addition, the amount of IFN-gamma in PBMC supernatants was significantly higher after vaccination. Overall, our results suggest the usefulness of evaluating the in vivo protective role of this promastigote preparation in dogs.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0147-9571
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
24
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
187-95
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:11440191-Animals,
pubmed-meshheading:11440191-Concanavalin A,
pubmed-meshheading:11440191-Cytotoxicity, Immunologic,
pubmed-meshheading:11440191-Disease Reservoirs,
pubmed-meshheading:11440191-Dog Diseases,
pubmed-meshheading:11440191-Dogs,
pubmed-meshheading:11440191-Female,
pubmed-meshheading:11440191-Humans,
pubmed-meshheading:11440191-Interferon-gamma,
pubmed-meshheading:11440191-Italy,
pubmed-meshheading:11440191-Leishmania infantum,
pubmed-meshheading:11440191-Leishmaniasis, Visceral,
pubmed-meshheading:11440191-Macrophage Activation,
pubmed-meshheading:11440191-Macrophages,
pubmed-meshheading:11440191-Male,
pubmed-meshheading:11440191-Nitric Oxide,
pubmed-meshheading:11440191-Phagocytosis,
pubmed-meshheading:11440191-Protozoan Vaccines,
pubmed-meshheading:11440191-Vaccination
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Nitric oxide production by macrophages of dogs vaccinated with killed Leishmania infantum promastigotes.
|
| pubmed:affiliation |
Dipartimento di Anatomia Umana e Istologia, University of Bari, Istloogia Policlinico, Italy. ma.panaro@anatomia.uniba.it
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|