Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
2001-7-4
pubmed:abstractText
Activation of the anaphase-promoting complex (APC) is required for anaphase initiation and for exit from mitosis in mammalian cells. Cdc20, which specifically recognizes APC substrates involved in the metaphase-to-anaphase transition, plays a pivotal role in APC activation through direct interaction with the APC. The activation of the APC by Cdc20 is prevented by the interaction of Cdc20 with Mad2 when the spindle checkpoint is activated. Using deletion mutagenesis and peptide mapping, we have identified the sequences in Cdc20 that target it to Mad2 and the APC, respectively. These sequences are distinct but overlapping, providing a possible structural explanation for the internal modulation of the APC-Cdc20 complex by Mad2. In the course of these studies, a truncation mutant of Cdc20 (1-153) that constitutively binds Mad2 but fails to bind the APC was identified. Overexpression of this mutant induces the formation of multinucleated cells and increases their susceptibility to undergoing apoptosis when treated with microtubule-inhibiting drugs. Our experiments demonstrate that disruption of the Mad2-Cdc20 interaction perturbs the mitotic checkpoint, leading to premature activation of the APC, sensitizing the cells to the cytotoxic effects of microtubule-inhibiting drugs.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-10072359, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-10202141, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-10228157, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-10329618, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-10352016, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-10403247, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-10436016, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-10477750, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-10597196, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-10647015, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-10700282, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-10827941, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-10892650, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-11030144, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-1423612, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-1651171, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-1651172, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-7730407, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-7736580, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-7787245, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-8567717, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-8601617, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-8688079, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-8824188, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-8824189, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-8939672, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-8985178, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-9005842, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-9182760, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-9288748, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-9334304, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-9461437, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-9461438, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-9482731, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-9546394, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-9635435, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-9637688, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-9660858, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-9695848, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-9736712, http://linkedlifedata.com/resource/pubmed/commentcorrection/11438673-9786942
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5190-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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