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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
35
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pubmed:dateCreated |
2001-8-27
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pubmed:abstractText |
Nef is a regulatory protein encoded by the genome of both human and simian immunodeficiency virus. Its expression in T cells leads to CD4 and major histocompatibility complex class I modulation and either enhancement or suppression of T cell activation. How this viral protein achieves multiple and at times opposing activities has been unclear. Through direct measurements of Nef and the Nef-GFP fusion protein, we find that these events are mediated by different Nef concentrations. Relative to the intracellular concentration that down-modulates surface CD4, an order of magnitude increase in Nef-GFP expression is required for a comparable modulation of major histocompatibility complex class I, and a further 3-fold increase is necessary to suppress T cell activation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
32763-70
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11438519-Antigens, CD4,
pubmed-meshheading:11438519-CD4-Positive T-Lymphocytes,
pubmed-meshheading:11438519-Cell Line,
pubmed-meshheading:11438519-Gene Expression Regulation,
pubmed-meshheading:11438519-Gene Expression Regulation, Viral,
pubmed-meshheading:11438519-Gene Products, nef,
pubmed-meshheading:11438519-Genes, MHC Class I,
pubmed-meshheading:11438519-Genes, Reporter,
pubmed-meshheading:11438519-Green Fluorescent Proteins,
pubmed-meshheading:11438519-HIV-1,
pubmed-meshheading:11438519-HLA-A Antigens,
pubmed-meshheading:11438519-Humans,
pubmed-meshheading:11438519-Jurkat Cells,
pubmed-meshheading:11438519-Luminescent Proteins,
pubmed-meshheading:11438519-Lymphocyte Activation,
pubmed-meshheading:11438519-Recombinant Fusion Proteins,
pubmed-meshheading:11438519-Simian immunodeficiency virus,
pubmed-meshheading:11438519-T-Lymphocytes,
pubmed-meshheading:11438519-nef Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2001
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pubmed:articleTitle |
HIV Nef-mediated cellular phenotypes are differentially expressed as a function of intracellular Nef concentrations.
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pubmed:affiliation |
Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, Maryland 20892-4034, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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