Linked Life Data

11437649

Source:http://linkedlifedata.com/resource/pubmed/id/11437649

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-7-4
pubmed:abstractText
UDP-glucuronosyltransferase 1A6 (UGT1A6), a key enzyme catalyzing the glucuronidation of small planar phenols and amines, is expressed in a tissue- and inducer-dependent manner. Expression is high in kidney, gastrointestinal tract, and induced liver, with low expression in spleen, lung, and ovary. Exposure to certain chemicals, such as 3-methylcholanthrene, benzo[a]pyrene, beta-naphthoflavone, and oltipraz elevates UGT1A6 mRNA in liver and to a lesser extent gastrointestinal tract and kidney, but not in other tissues. The mechanisms underlying this complex pattern of expression have been elusive. We have identified a new type of UGT1A6 mRNA (class 2) that differs in its 5' untranslated sequence. The class 2 transcript is the more abundant type expressed in liver, gastrointestinal tract, and kidney. Transcription of the class 2 mRNA is initiated 107 bases 5' of the UGT1A6 coding exon. The promoter region flanking the transcription start site contains an HNF1-like binding site identical to that in the human UGT1A6 gene. Both class 1 and class 2 mRNAs were elevated in liver by 3-methylcholanthrene, benzo[a]pyrene, beta-naphthoflavone, and oltipraz, with preferential elevation of class 1 occurring after 3-methylcholanthrene and benzo[a]pyrene treatment. These data suggest that transcription from a second promoter contributes to tissue- and inducer-specific expression of rat UGT1A6.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0041-008X
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Academic Press.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
174
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
60-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11437649-5' Untranslated Regions, pubmed-meshheading:11437649-Animals, pubmed-meshheading:11437649-Base Sequence, pubmed-meshheading:11437649-Benzo(a)pyrene, pubmed-meshheading:11437649-DNA, Complementary, pubmed-meshheading:11437649-Digestive System, pubmed-meshheading:11437649-Exons, pubmed-meshheading:11437649-Gene Expression, pubmed-meshheading:11437649-Glucuronosyltransferase, pubmed-meshheading:11437649-Humans, pubmed-meshheading:11437649-Kidney, pubmed-meshheading:11437649-Liver, pubmed-meshheading:11437649-Male, pubmed-meshheading:11437649-Methylcholanthrene, pubmed-meshheading:11437649-Molecular Sequence Data, pubmed-meshheading:11437649-Promoter Regions, Genetic, pubmed-meshheading:11437649-Pyrazines, pubmed-meshheading:11437649-RNA, Messenger, pubmed-meshheading:11437649-Rats, pubmed-meshheading:11437649-Rats, Sprague-Dawley, pubmed-meshheading:11437649-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11437649-Sequence Alignment, pubmed-meshheading:11437649-beta-Naphthoflavone
pubmed:year
2001
pubmed:articleTitle
An alternative promoter contributes to tissue- and inducer-specific expression of the rat UDP-glucuronosyltransferase 1A6 gene.
pubmed:affiliation
Department of Pharmacology and Toxicology, Medical College of Virginia Campus of Virginia Commonwealth University, Richmond, Virginia 23298, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't