| pubmed-article:11437276 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11437276 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:11437276 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:11437276 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:11437276 | lifeskim:mentions | umls-concept:C0042036 | lld:lifeskim |
| pubmed-article:11437276 | lifeskim:mentions | umls-concept:C0018183 | lld:lifeskim |
| pubmed-article:11437276 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:11437276 | lifeskim:mentions | umls-concept:C1521761 | lld:lifeskim |
| pubmed-article:11437276 | lifeskim:mentions | umls-concept:C1948023 | lld:lifeskim |
| pubmed-article:11437276 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:11437276 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:11437276 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:11437276 | pubmed:dateCreated | 2001-7-4 | lld:pubmed |
| pubmed-article:11437276 | pubmed:abstractText | We have generated transgenic mice expressing human granulocyte macrophage-colony stimulating factor (hGM-CSF) in urine. In particular, the expression plasmid DNA containing mouse uroplakin II promoter was used to direct uroepithelium-specific transcription of transgene. In this study, hGM-CSF transcript was detected only in bladder uroepithelium as determined by northern blot analysis. Furthermore, hGM-CSF protein was detected in the suprabasal layer of the uroepithelium and ureter by immunohistochemistry. The hGM-CSF was secreted into urine at high level (up to 180 ng/ml), and enhanced proliferation of hGM-CSF-dependent human acute monocyte leukemic cells, suggesting that transgenic urine-derived hGM-CSF was bioactive. This is the first case of demonstrating biological activity of a cytokine produced in the urine of a transgenic animal. Our results demonstrate that bladder can be used as a bioreactor to produce biologically important substances. In addition, it suggests a potential application of bladder expression system to livestock for high-yield production of pharmaceuticals. | lld:pubmed |
| pubmed-article:11437276 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11437276 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11437276 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11437276 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11437276 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11437276 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11437276 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11437276 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11437276 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11437276 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11437276 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11437276 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:11437276 | pubmed:issn | 0962-8819 | lld:pubmed |
| pubmed-article:11437276 | pubmed:author | pubmed-author:LeeJ WJW | lld:pubmed |
| pubmed-article:11437276 | pubmed:author | pubmed-author:KimJ HJH | lld:pubmed |
| pubmed-article:11437276 | pubmed:author | pubmed-author:KimK EKE | lld:pubmed |
| pubmed-article:11437276 | pubmed:author | pubmed-author:ByunS JSJ | lld:pubmed |
| pubmed-article:11437276 | pubmed:author | pubmed-author:ParkS HSH | lld:pubmed |
| pubmed-article:11437276 | pubmed:author | pubmed-author:JinS ZSZ | lld:pubmed |
| pubmed-article:11437276 | pubmed:author | pubmed-author:HwangH YHY | lld:pubmed |
| pubmed-article:11437276 | pubmed:author | pubmed-author:HaqJ AJA | lld:pubmed |
| pubmed-article:11437276 | pubmed:author | pubmed-author:RuffV AVA | lld:pubmed |
| pubmed-article:11437276 | pubmed:author | pubmed-author:YounJJ | lld:pubmed |
| pubmed-article:11437276 | pubmed:author | pubmed-author:BahkY YYY | lld:pubmed |
| pubmed-article:11437276 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11437276 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:11437276 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11437276 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11437276 | pubmed:pagination | 193-200 | lld:pubmed |
| pubmed-article:11437276 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:11437276 | pubmed:meshHeading | pubmed-meshheading:11437276... | lld:pubmed |
| pubmed-article:11437276 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11437276 | pubmed:articleTitle | Expression of recombinant human granulocyte macrophage-colony stimulating factor (hGM-CSF) in mouse urine. | lld:pubmed |
| pubmed-article:11437276 | pubmed:affiliation | Department of Immunobiology and Dermatology, College of Medicine, Catholic University of Korea, Seoul. | lld:pubmed |
| pubmed-article:11437276 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11437276 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11437276 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11437276 | lld:pubmed |
