|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2001-7-4
|
|
pubmed:abstractText |
We have generated transgenic mice expressing human granulocyte macrophage-colony stimulating factor (hGM-CSF) in urine. In particular, the expression plasmid DNA containing mouse uroplakin II promoter was used to direct uroepithelium-specific transcription of transgene. In this study, hGM-CSF transcript was detected only in bladder uroepithelium as determined by northern blot analysis. Furthermore, hGM-CSF protein was detected in the suprabasal layer of the uroepithelium and ureter by immunohistochemistry. The hGM-CSF was secreted into urine at high level (up to 180 ng/ml), and enhanced proliferation of hGM-CSF-dependent human acute monocyte leukemic cells, suggesting that transgenic urine-derived hGM-CSF was bioactive. This is the first case of demonstrating biological activity of a cytokine produced in the urine of a transgenic animal. Our results demonstrate that bladder can be used as a bioreactor to produce biologically important substances. In addition, it suggests a potential application of bladder expression system to livestock for high-yield production of pharmaceuticals.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0962-8819
|
|
pubmed:author |
pubmed-author:BahkY YYY,
pubmed-author:ByunS JSJ,
pubmed-author:HaqJ AJA,
pubmed-author:HwangH YHY,
pubmed-author:JinS ZSZ,
pubmed-author:KimJ HJH,
pubmed-author:KimK EKE,
pubmed-author:LeeJ WJW,
pubmed-author:ParkS HSH,
pubmed-author:RuffV AVA,
pubmed-author:YounJJ
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
10
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
193-200
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:11437276-Animals,
pubmed-meshheading:11437276-Base Sequence,
pubmed-meshheading:11437276-Biotechnology,
pubmed-meshheading:11437276-Cell Division,
pubmed-meshheading:11437276-Cell Line,
pubmed-meshheading:11437276-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11437276-Epithelium,
pubmed-meshheading:11437276-Gene Expression,
pubmed-meshheading:11437276-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:11437276-Humans,
pubmed-meshheading:11437276-Immunohistochemistry,
pubmed-meshheading:11437276-Membrane Proteins,
pubmed-meshheading:11437276-Mice,
pubmed-meshheading:11437276-Mice, Transgenic,
pubmed-meshheading:11437276-Organ Specificity,
pubmed-meshheading:11437276-Promoter Regions, Genetic,
pubmed-meshheading:11437276-RNA, Messenger,
pubmed-meshheading:11437276-Recombinant Proteins,
pubmed-meshheading:11437276-Transgenes,
pubmed-meshheading:11437276-Urinary Bladder,
pubmed-meshheading:11437276-Uroplakin II
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
Expression of recombinant human granulocyte macrophage-colony stimulating factor (hGM-CSF) in mouse urine.
|
|
pubmed:affiliation |
Department of Immunobiology and Dermatology, College of Medicine, Catholic University of Korea, Seoul.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
