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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5-6
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pubmed:dateCreated |
2001-7-4
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pubmed:abstractText |
Two distinct classes of lipocalin isoforms (OBP-IIs and OBP-IIIs) were purified and identified from porcine nasal mucosa of male and female individuals. Using primers designed on their N-terminal sequence, the complete primary structures of the mature polypeptides were determined. Mass spectrometry analysis confirmed the identity of the cDNA-derived sequences and provided information regarding their post-translational modifications. These species strongly resemble a lipocalin expressed by von Ebner's gland and salivary lipocalins carrying sex-specific pheromones secreted only by the boar's submaxillary glands. Both OBP-IIs and OBP-IIIs present two cysteines paired in a disulphide bond; the remaining residues occur in a reduced form. In addition, OBP-IIIs are heavily glycosylated and markedly different in their glycan moiety from the salivary lipocalins. A three-dimensional model is proposed based on protein species with known structure. Like salivary lipocalins, OBP-IIIs bind a number of odorant molecules, with highest affinity for the specific pheromone 5alpha-androst-16-en-3-one. The high similarity between OBPs from the nasal area and lipocalins from secretory glands suggests a common function in binding the same pheromonal ligands, the latter carrying chemical messages into the environment the former delivering them to specific receptors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Androstenes,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Insect Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Lipocalins,
http://linkedlifedata.com/resource/pubmed/chemical/Pheromones,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Odorant,
http://linkedlifedata.com/resource/pubmed/chemical/Salivary Proteins and Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/androst-16-en-3-one,
http://linkedlifedata.com/resource/pubmed/chemical/odorant-binding protein,
http://linkedlifedata.com/resource/pubmed/chemical/pheromone binding protein, insect,
http://linkedlifedata.com/resource/pubmed/chemical/salivary lipocalin protein, Sus...
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1420-682X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
58
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
823-34
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11437241-Amino Acid Sequence,
pubmed-meshheading:11437241-Androstenes,
pubmed-meshheading:11437241-Animals,
pubmed-meshheading:11437241-Carrier Proteins,
pubmed-meshheading:11437241-Cloning, Molecular,
pubmed-meshheading:11437241-Epithelium,
pubmed-meshheading:11437241-Female,
pubmed-meshheading:11437241-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:11437241-Glycosylation,
pubmed-meshheading:11437241-Insect Proteins,
pubmed-meshheading:11437241-Ligands,
pubmed-meshheading:11437241-Lipocalins,
pubmed-meshheading:11437241-Male,
pubmed-meshheading:11437241-Models, Molecular,
pubmed-meshheading:11437241-Molecular Sequence Data,
pubmed-meshheading:11437241-Olfactory Mucosa,
pubmed-meshheading:11437241-Pheromones,
pubmed-meshheading:11437241-Protein Conformation,
pubmed-meshheading:11437241-Receptors, Odorant,
pubmed-meshheading:11437241-Salivary Proteins and Peptides,
pubmed-meshheading:11437241-Sequence Analysis,
pubmed-meshheading:11437241-Spectrometry, Mass, Matrix-Assisted Laser...
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pubmed:year |
2001
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pubmed:articleTitle |
Purification, cloning and characterisation of odorant- and pheromone-binding proteins from pig nasal epithelium.
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pubmed:affiliation |
I.A.B.B.A.M.-Centro Internazionale Servizi di Spettrometria di Massa National Research Council, Napoli, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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