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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
2001-7-3
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pubmed:abstractText |
The human immunodeficiency virus type 1 (HIV-1) nucleocapsid mutation R10A/K11A abolishes viral replication when present in proviral clone HIV-1(HXB-2), but it was found to have minimal effect on replication of the closely related HIV-1(NL4-3). Functional mapping demonstrated that a nonconservative amino acid change at nucleocapsid residue 24 (threonine in HIV-1(HXB-2), isoleucine in HIV-1(NL4-3)) is the major determinant of the different R10A/K11A phenotypes in these two proviruses. Threonine-isoleucine exchanges appear to modify the R10A/K11A phenotype via effects on virion RNA-packaging efficiency. The improved packaging seen with hydrophobic isoleucine is consistent with solution structures localizing this residue to a hydrophobic pocket that contacts guanosine bases in viral genomic RNA stem-loops critical for packaging.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-10364286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-10400764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-10482569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-10708419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-10756042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-10823883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-10888611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-10926523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-1548759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-2410792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-6327821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-8032280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-8791728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-8794295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-9223522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-9430589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-9632380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-9658109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-9757830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11435601-9922136
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7193-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11435601-Amino Acid Sequence,
pubmed-meshheading:11435601-Chromosome Mapping,
pubmed-meshheading:11435601-HIV-1,
pubmed-meshheading:11435601-Humans,
pubmed-meshheading:11435601-Molecular Sequence Data,
pubmed-meshheading:11435601-Mutagenesis,
pubmed-meshheading:11435601-Nucleocapsid,
pubmed-meshheading:11435601-Phenotype,
pubmed-meshheading:11435601-RNA, Viral,
pubmed-meshheading:11435601-Virion,
pubmed-meshheading:11435601-Virus Assembly
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pubmed:year |
2001
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pubmed:articleTitle |
Context-dependent phenotype of a human immunodeficiency virus type 1 nucleocapsid mutation.
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pubmed:affiliation |
Department of Microbiology, College of Physicians and Surgeons, Columbia Univeirsity, New York, New York, 10032, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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