Source:http://linkedlifedata.com/resource/pubmed/id/11430461
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2001-6-29
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pubmed:abstractText |
Simultaneous recording of Ca2+-images in one confocal plane from vascular smooth muscle cells (SMCs) and endothelial cells (ECs) of an intact rat femoral artery segment was performed using indo-1 and a confocal microscope. During application of 10 microM acetylcholine (ACh), elevation and oscillation of intracellular Ca2+ concentration ([Ca2+]i) were observed in ECs but not in SMCs. Sequential conduction of Ca2+ oscillation from an EC to the neighboring ECs in one longitudinal direction was often observed in the presence of ACh. On the other hand, the activation of voltage-dependent Ca2+ channels by external 30 mM K+ resulted in the elevation of [Ca2+]i only in SMCs. When 10 microM ACh was added in the presence of 30 mM K+, it was observed in one confocal plane that [Ca2+]i in ECs and SMCs was almost simultaneously increased and decreased, respectively. The simultaneous recording method in this intact preparation will provide a line of valuable information about the interactions between SMCs and ECs, based on spatio-temporal analyses of absolute values of [Ca2+]i in individual cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-5198
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
106-13
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:11430461-Acetylcholine,
pubmed-meshheading:11430461-Animals,
pubmed-meshheading:11430461-Calcium,
pubmed-meshheading:11430461-Endothelium, Vascular,
pubmed-meshheading:11430461-Femoral Artery,
pubmed-meshheading:11430461-Image Processing, Computer-Assisted,
pubmed-meshheading:11430461-Male,
pubmed-meshheading:11430461-Microscopy, Confocal,
pubmed-meshheading:11430461-Microscopy, Fluorescence,
pubmed-meshheading:11430461-Muscle, Smooth, Vascular,
pubmed-meshheading:11430461-Rats,
pubmed-meshheading:11430461-Rats, Wistar
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pubmed:year |
2001
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pubmed:articleTitle |
Ca2+-images of smooth muscle cells and endothelial cells in one confocal plane in femoral artery segments of the rat.
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pubmed:affiliation |
Department of Molecular and Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.
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pubmed:publicationType |
Journal Article
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