Source:http://linkedlifedata.com/resource/pubmed/id/11429469
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
Pt 7
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| pubmed:dateCreated |
2001-6-28
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| pubmed:abstractText |
Shiga-toxin-2 (stx(2))-encoding bacteriophages were isolated from Norwegian Escherichia coli O157:H7 isolates of cattle and human origin. The phages were characterized by restriction enzyme analysis, hybridization with probes for toxin genes and selected phage DNA such as the P gene, integrase gene and IS1203, and by PCR studies and partial sequencing of selected DNA regions in the integrase to stx(2) region of the phages. The stx(2)-phage-containing bacteria from which inducible phages were detected produced similar amounts of toxin, as shown by a Vero cell assay. The results indicate that the population of stx(2)-carrying phages is heterogeneous, although the phages from epidemiologically linked E. coli O157:H7 isolates were similar. There appears to have been frequent recombination of stx(2) phages with other lambdoid phages.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
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| pubmed:issn |
1350-0872
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
147
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1929-36
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:11429469-Animals,
pubmed-meshheading:11429469-Bacteriophage lambda,
pubmed-meshheading:11429469-Cattle,
pubmed-meshheading:11429469-Cattle Diseases,
pubmed-meshheading:11429469-Cercopithecus aethiops,
pubmed-meshheading:11429469-Child, Preschool,
pubmed-meshheading:11429469-Coliphages,
pubmed-meshheading:11429469-DNA, Viral,
pubmed-meshheading:11429469-Escherichia coli Infections,
pubmed-meshheading:11429469-Escherichia coli O157,
pubmed-meshheading:11429469-Female,
pubmed-meshheading:11429469-Gene Transfer, Horizontal,
pubmed-meshheading:11429469-Genetic Variation,
pubmed-meshheading:11429469-Genome, Viral,
pubmed-meshheading:11429469-Humans,
pubmed-meshheading:11429469-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:11429469-Recombination, Genetic,
pubmed-meshheading:11429469-Shiga Toxin 2,
pubmed-meshheading:11429469-Vero Cells,
pubmed-meshheading:11429469-Virus Activation
|
| pubmed:year |
2001
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| pubmed:articleTitle |
Mosaic structure of Shiga-toxin-2-encoding phages isolated from Escherichia coli O157:H7 indicates frequent gene exchange between lambdoid phage genomes.
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| pubmed:affiliation |
Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School of Veterinary Science, PO Box 8146 Dep., N-0033 Oslo, Norway.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|