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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2001-6-27
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pubmed:abstractText |
Cytokines and growth factors are indispensable for the propagation and maintenance of factor-dependent mammalian cells. However, cytokines are often so expensive that the use of factor-dependent cells for industrial applications such as protein production is often not practical. Based on our previous design of a binary hen egg lysozyme (HEL)-specific receptor composed of portions of the anti-HEL antibody and the erythropoietin receptor, a new pair of chimeric receptors having the intracellular domain of gp130 were made and transfected to an interleukin-6 (IL-6)-dependent hybridoma, 7TD1. The clone expressing the two new receptors showed clear HEL dose-dependent cell growth and monoclonal antibody production in both serum-based and serum-free media without IL-6. These results establish the feasibility of applying receptor design to tailor cells for the inexpensive induction of cell growth for the purpose of producing therapeutic products.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Muramidase,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Stat3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/hen egg lysozyme
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-3592
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 John Wiley & Sons, Inc.
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
416-23
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11427943-Animals,
pubmed-meshheading:11427943-Antibodies, Monoclonal,
pubmed-meshheading:11427943-Cell Division,
pubmed-meshheading:11427943-Cell Line,
pubmed-meshheading:11427943-Culture Media, Serum-Free,
pubmed-meshheading:11427943-DNA-Binding Proteins,
pubmed-meshheading:11427943-Efficiency,
pubmed-meshheading:11427943-Hybridomas,
pubmed-meshheading:11427943-Interleukin-6,
pubmed-meshheading:11427943-Mice,
pubmed-meshheading:11427943-Muramidase,
pubmed-meshheading:11427943-Phosphorylation,
pubmed-meshheading:11427943-Receptors, Cell Surface,
pubmed-meshheading:11427943-Recombinant Fusion Proteins,
pubmed-meshheading:11427943-STAT3 Transcription Factor,
pubmed-meshheading:11427943-Trans-Activators,
pubmed-meshheading:11427943-Transfection
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pubmed:year |
2001
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pubmed:articleTitle |
Replacing factor-dependency with that for lysozyme: affordable culture of IL-6-dependent hybridoma by transfecting artificial cell surface receptor.
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pubmed:affiliation |
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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