|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
7
|
|
pubmed:dateCreated |
2001-6-27
|
|
pubmed:abstractText |
Susceptibility testing of fungi by flow cytometry (also called fluorescence-activated cell sorting [FACS]) using vital staining with FUN-1 showed a good correlation with the standard M27-A procedure for assessing MICs. In this study we determined MICs for blood culture isolates from patients with candidemia by NCCLS M27-A and FACS methods and correlated the clinical outcome of these patients with in vitro antifungal resistance test results. A total of 24 patients with candidemia for whom one or more blood cultures were positive for a Candida sp. were included. Susceptibility testing was performed by NCCLS M27-A and FACS methods. The correlation of MICs (NCCLS M27-A and FACS) and clinical outcome was calculated. In 83% of the cases, the MICs of fluconazole determined by FACS were within 1 dilution of the MICs determined by the NCCLS M27-A method. For proposed susceptibility breakpoints, there was 100% agreement between the M27-A and FACS methods. In the FACS assay, a fluconazole MIC of <1 microg/ml was associated with cure (P < 0.001) whereas an MIC of > or =1 microg/ml was associated with death (P < 0.001). The M27-A-derived fluconazole MICs did not correlate with outcome (P = 1 and P = 0.133).
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-10471550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-10770728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-10991850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-10991856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-1669837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-2896743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-3284339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-3517051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-3674846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-6520227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-7695326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-7785993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-8027319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-8035024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-8269392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-8473760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-8904400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-8968873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-9003596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-9114154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-9228472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-9276410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-9593135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11427554-9624506
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0095-1137
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
39
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2458-62
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:11427554-Adolescent,
pubmed-meshheading:11427554-Adult,
pubmed-meshheading:11427554-Aged,
pubmed-meshheading:11427554-Aged, 80 and over,
pubmed-meshheading:11427554-Antifungal Agents,
pubmed-meshheading:11427554-Blood,
pubmed-meshheading:11427554-Candida,
pubmed-meshheading:11427554-Candidiasis,
pubmed-meshheading:11427554-Culture Media,
pubmed-meshheading:11427554-Drug Resistance, Microbial,
pubmed-meshheading:11427554-Female,
pubmed-meshheading:11427554-Flow Cytometry,
pubmed-meshheading:11427554-Fluconazole,
pubmed-meshheading:11427554-Fungemia,
pubmed-meshheading:11427554-Humans,
pubmed-meshheading:11427554-Infant,
pubmed-meshheading:11427554-Male,
pubmed-meshheading:11427554-Microbial Sensitivity Tests,
pubmed-meshheading:11427554-Middle Aged,
pubmed-meshheading:11427554-Treatment Outcome
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
Antifungal susceptibility testing of fluconazole by flow cytometry correlates with clinical outcome.
|
|
pubmed:affiliation |
Division of Infectious Diseases, Department of Medicine, Karl-Franzens University, Auenbruggerplatz 15, A-8036 Graz, Austria. christoph.wenisch@kfunigraz.ac.at
|
|
pubmed:publicationType |
Journal Article
|
