Linked Life Data

11425891

Source:http://linkedlifedata.com/resource/pubmed/id/11425891

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
2001-6-26
pubmed:databankReference
pubmed:abstractText
Reporter gene expression in the olfactory epithelium of H-lacZ6 transgenic mice mimics the cell-selective expression pattern known for some odorant receptor genes. The transgene construct in these mice consists of the lacZ coding region, driven by the proximal olfactory marker protein (OMP) gene promoter, and shows expression in a zonally confined subpopulation of olfactory neurons. To address mechanisms underlying the odorant receptor-like expression pattern of the lacZ construct, we analyzed the transgene-flanking region and identified OR-Z6, the first cloned odorant receptor gene that maps to mouse chromosome 6. OR-Z6 bears the highest sequence similarity (85%) to a human odorant receptor gene at the syntenic location on human chromosome 7. We analyzed the expression pattern of OR-Z6 in olfactory tissues of H-lacZ6 mice and show that it bears strong similarities to that mapped for beta-galactosidase. Expression of both genes in olfactory neurons is primarily restricted to the same medial subregion of the olfactory epithelium. Axons from both neuronal subpopulations project to the same ventromedial aspect of the anterior olfactory bulbs. Furthermore, colocalization analyses in H-lacZ6 mice demonstrate that OR-Z6-reactive glomeruli receive axonal input from lacZ-positive neurons as well. These results suggest that the expression of both genes is coordinated and that transgene expression in H-lacZ6 mice is regulated by locus-dependent mechanisms.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4637-48
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11425891-Animals, pubmed-meshheading:11425891-Axons, pubmed-meshheading:11425891-Chromosome Mapping, pubmed-meshheading:11425891-Chromosomes, pubmed-meshheading:11425891-Chromosomes, Human, Pair 7, pubmed-meshheading:11425891-Gene Expression Regulation, pubmed-meshheading:11425891-Genes, Reporter, pubmed-meshheading:11425891-Genomic Library, pubmed-meshheading:11425891-Humans, pubmed-meshheading:11425891-In Situ Hybridization, pubmed-meshheading:11425891-Mice, pubmed-meshheading:11425891-Mice, Inbred Strains, pubmed-meshheading:11425891-Mice, Transgenic, pubmed-meshheading:11425891-Molecular Sequence Data, pubmed-meshheading:11425891-Multigene Family, pubmed-meshheading:11425891-Nerve Tissue Proteins, pubmed-meshheading:11425891-Olfactory Bulb, pubmed-meshheading:11425891-Olfactory Marker Protein, pubmed-meshheading:11425891-Olfactory Mucosa, pubmed-meshheading:11425891-Olfactory Receptor Neurons, pubmed-meshheading:11425891-Organ Specificity, pubmed-meshheading:11425891-Promoter Regions, Genetic, pubmed-meshheading:11425891-Receptors, Odorant, pubmed-meshheading:11425891-Sequence Homology, Nucleic Acid, pubmed-meshheading:11425891-Transgenes, pubmed-meshheading:11425891-beta-Galactosidase
pubmed:year
2001
pubmed:articleTitle
The OMP-lacZ transgene mimics the unusual expression pattern of OR-Z6, a new odorant receptor gene on mouse chromosome 6: implication for locus-dependent gene expression.
pubmed:affiliation
Department of Anatomy and Neurobiology, School of Medicine, University of Maryland at Baltimore, Baltimore, Maryland 21201, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.