Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2001-6-26
pubmed:abstractText
The enzyme glutamate decarboxylase (GAD) is prevalent in Escherichia coli but few strains in the various pathogenic E. coli groups have been tested for GAD. Using PCR primers that amplify a 670-bp segment from the gadA and gadB genes encoding GAD, we examined the distribution of the gadAB genes among enteric bacteria. Analysis of 173 pathogenic E. coli strains, including 125 enterohemorrhagic E. coli isolates of the O157:H7 serotype and its phenotypic variants and 48 isolates of enteropathogenic E. coli, enterotoxigenic E. coli, enteroinvasive E. coli, and other Shiga toxin-producing E. coli (STEC) serotypes, showed that gadAB genes were present in all these strains. Among the 22 non-E. coli isolates tested, only the 6 Shigella spp. carried gadAB. Analysis of naturally contaminated water and food samples using a gadAB-specific DNA probe that was labeled with digoxigenin showed that a gadAB-based assay is as reliable as standard methods that enumerate E. coli organisms on the basis of lactose fermentation. The presence of few E. coli cells initially seeded into produce rinsates could be detected by PCR to gadA/B genes after overnight enrichment. A multiplex PCR assay using the gadAB primers in combination with primers to Shiga toxin (Stx) genes stx(1) and stx(2) was effective in detecting STEC from the enrichment medium after seeding produce rinsate samples with as few as 2 CFU. The gadAB primers may be multiplexed with primers to other trait virulence markers to specifically identify other pathogenic E. coli groups.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-10340661, http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-10549421, http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-10792533, http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-10983788, http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-1522060, http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-1768116, http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-6391379, http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-7016033, http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-7608084, http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-7921260, http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-8285721, http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-8586736, http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-8795195, http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-8795225, http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-9114382, http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-9293003, http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-9457432, http://linkedlifedata.com/resource/pubmed/commentcorrection/11425729-9666018
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3110-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Glutamate decarboxylase genes as a prescreening marker for detection of pathogenic Escherichia coli groups.
pubmed:affiliation
U.S. Food and Drug Administration, Pacific Regional Laboratory-Northwest, Bothell, Washington 98021-4421, USA.
pubmed:publicationType
Journal Article