Linked Life Data

11420249

Source:http://linkedlifedata.com/resource/pubmed/id/11420249

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-6-22
pubmed:abstractText
During the periovulatory period, the mammalian ovary is the site of dramatic functional and structural changes, leading to oocyte maturation, follicle rupture, and corpus luteum formation. To a large extent, these processes result from changes in the transcriptome of various ovarian cell types. To develop a broader view of periovulatory changes in gene expression in the ovary and to identify further genes involved in periovulatory events, we used the recently developed DNA array technology. Immature female eCG-primed rats were killed either immediately before or 6 h after ovulation induction with hCG. Total ovarian RNA was isolated and used to prepare radiolabeled cDNA probes, which were hybridized to DNA arrays representing approximately 600 rat genes. Quantitative analysis identified a multitude of regulated gene messages, including several genes involved in extracellular matrix degradation and lipid/steroid metabolism previously reported to be induced by hCG. This screening also identified a group of candidate genes whose ovarian expression and gonadotropin regulation was hitherto unknown. The induction of three of these genes, encoding cutaneous fatty acid-binding protein, the interleukin-4 receptor alpha chain, and prepronociceptin, was confirmed and further characterized by Northern blot analysis. In addition, in situ hybridization analysis showed that hCG administration resulted in exclusive or predominant expression of all three genes in theca cells. These results demonstrate that DNA arrays can be used to identify genes regulated during the periovulatory period, thus contributing to a more detailed understanding of the molecular mechanisms of ovulation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
269-76
pubmed:dateRevised
2011-10-3
pubmed:meshHeading
pubmed-meshheading:11420249-Animals, pubmed-meshheading:11420249-Blotting, Northern, pubmed-meshheading:11420249-Carrier Proteins, pubmed-meshheading:11420249-DNA, pubmed-meshheading:11420249-DNA Probes, pubmed-meshheading:11420249-Fatty Acid-Binding Proteins, pubmed-meshheading:11420249-Female, pubmed-meshheading:11420249-Gene Expression Regulation, pubmed-meshheading:11420249-Gonadotropins, pubmed-meshheading:11420249-In Situ Hybridization, pubmed-meshheading:11420249-Neoplasm Proteins, pubmed-meshheading:11420249-Nerve Tissue Proteins, pubmed-meshheading:11420249-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:11420249-Ovary, pubmed-meshheading:11420249-Ovulation, pubmed-meshheading:11420249-Rats, pubmed-meshheading:11420249-Rats, Sprague-Dawley, pubmed-meshheading:11420249-Receptors, Interleukin-4
pubmed:year
2001
pubmed:articleTitle
DNA array analysis of changes in preovulatory gene expression in the rat ovary.
pubmed:affiliation
Division of Reproductive Biology, Department of Gynecology and Obstetrics, Stanford University Medical Center, Stanford, California 94305-5317, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't