|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2001-6-22
|
|
pubmed:abstractText |
The amiloride-inhibitable Na(+)/H(+) antiporter plays an important role in macrophage activation. The intracellular pathways leading to interleukin (IL)-12 p40 production by activated macrophages are incompletely understood. In the present study, we examined the contribution of the Na(+)/H(+) antiporter to the production of IL-12 p40. Amiloride or its analogs decreased the production of IL-12 p40 in macrophages stimulated with bacterial lipopolysaccharide and interferon-gamma. The order of potency of amiloride analogs was consistent with the proposition that the effect of amiloride is mediated by the inhibition of the Na(+)/H(+) antiporter. The effect of amiloride was post-transcriptional, as IL-12 p40 mRNA levels induced by lipopolysaccharide and interferon-gamma were not affected by this inhibitor. Furthermore, the inhibitory effect of amiloride on IL-12 p40 production was not a result of interference with the activation of the p38 and p42/44 mitogen-activated protein kinases or c-Jun kinase. In summary, the production of IL-12 p40 requires a functional Na(+)/H(+) antiporter.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5-dimethylamiloride,
http://linkedlifedata.com/resource/pubmed/chemical/Amiloride,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL4,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL2,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines,
http://linkedlifedata.com/resource/pubmed/chemical/Cxcl2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Diuretics,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Inflammatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Methacrylates,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/N-(4-(2-(4-isobutylphenyl)propionylo...,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Hydrogen Antiporter,
http://linkedlifedata.com/resource/pubmed/chemical/benzamil,
http://linkedlifedata.com/resource/pubmed/chemical/ethylisopropylamiloride,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0006-3002
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
20
|
|
pubmed:volume |
1539
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
233-42
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:11420121-Amiloride,
pubmed-meshheading:11420121-Animals,
pubmed-meshheading:11420121-Chemokine CCL4,
pubmed-meshheading:11420121-Chemokine CXCL2,
pubmed-meshheading:11420121-Chemokines,
pubmed-meshheading:11420121-Diuretics,
pubmed-meshheading:11420121-Drug Interactions,
pubmed-meshheading:11420121-Enzyme Activation,
pubmed-meshheading:11420121-Gene Silencing,
pubmed-meshheading:11420121-Interleukin-12,
pubmed-meshheading:11420121-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:11420121-Lipopolysaccharides,
pubmed-meshheading:11420121-Macrophage Inflammatory Proteins,
pubmed-meshheading:11420121-Macrophages, Peritoneal,
pubmed-meshheading:11420121-Male,
pubmed-meshheading:11420121-Methacrylates,
pubmed-meshheading:11420121-Mice,
pubmed-meshheading:11420121-Mice, Inbred BALB C,
pubmed-meshheading:11420121-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:11420121-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:11420121-Mitogen-Activated Protein Kinases,
pubmed-meshheading:11420121-Phosphorylation,
pubmed-meshheading:11420121-Sodium-Hydrogen Antiporter,
pubmed-meshheading:11420121-p38 Mitogen-Activated Protein Kinases
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
Inhibition of the Na(+)/H(+) antiporter suppresses IL-12 p40 production by mouse macrophages.
|
|
pubmed:affiliation |
Department of Surgery, UMD-New Jersey Medical School, Newark, NJ 07103, USA.
|
|
pubmed:publicationType |
Journal Article
|
