Linked Life Data

11419941

Source:http://linkedlifedata.com/resource/pubmed/id/11419941

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-6-22
pubmed:databankReference
pubmed:abstractText
Multiple extracellular factors are hypothesized to promote the differentiation of unstimulated and/or stimulated secretory pathways in exocrine secretory cells, but the identity of differentiation factors, particularly those organ-specific, remain largely unknown. Here, we report on the identification of a novel secreted glycoprotein, lacritin, that enhances exocrine secretion in overnight cultures of lacrimal acinar cells which otherwise display loss of secretory function. Lacritin mRNA and protein are highly expressed in human lacrimal gland, moderately in major and minor salivary glands and slightly in thyroid. No lacritin message or protein is detected elsewhere among more than 50 human tissues examined. Lacritin displays partial similarity to the glycosaminoglycan-binding region of brain-specific neuroglycan C (32 % identity over 102 amino acid residues) and to the possibly mucin-like amino globular region of fibulin-2 (30 % identity over 81 amino acid residues), and localizes primarily to secretory granules and secretory fluid. The lacritin gene consists of five exons, displays no alternative splicing and maps to 12q13. Recombinant lacritin augments unstimulated but not stimulated acinar cell secretion, promotes ductal cell proliferation, and stimulates signaling through tyrosine phosphorylation and release of calcium. It binds collagen IV, laminin-1, entactin/nidogen-1, fibronectin and vitronectin, but not collagen I, heparin or EGF. As an autocrine/paracrine enhancer of the lacrimal constitutive secretory pathway, ductal cell mitogen and stimulator of corneal epithelial cells, lacritin may play a key role in the function of the lacrimal gland-corneal axis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-2836
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Academic Press.
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
310
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
127-39
pubmed:dateRevised
2011-2-4
pubmed:meshHeading
pubmed-meshheading:11419941-Amino Acid Sequence, pubmed-meshheading:11419941-Animals, pubmed-meshheading:11419941-Base Sequence, pubmed-meshheading:11419941-Calcium Signaling, pubmed-meshheading:11419941-Cell Division, pubmed-meshheading:11419941-Cells, Cultured, pubmed-meshheading:11419941-Chromosomes, Human, Pair 12, pubmed-meshheading:11419941-Cloning, Molecular, pubmed-meshheading:11419941-Cornea, pubmed-meshheading:11419941-DNA, Complementary, pubmed-meshheading:11419941-Epithelial Cells, pubmed-meshheading:11419941-Exons, pubmed-meshheading:11419941-Extracellular Matrix Proteins, pubmed-meshheading:11419941-Genomic Library, pubmed-meshheading:11419941-Glycoproteins, pubmed-meshheading:11419941-Humans, pubmed-meshheading:11419941-In Situ Hybridization, Fluorescence, pubmed-meshheading:11419941-Lacrimal Apparatus, pubmed-meshheading:11419941-Membrane Proteins, pubmed-meshheading:11419941-Molecular Sequence Data, pubmed-meshheading:11419941-Organ Specificity, pubmed-meshheading:11419941-Phosphorylation, pubmed-meshheading:11419941-Proteoglycans, pubmed-meshheading:11419941-RNA, Messenger, pubmed-meshheading:11419941-RNA Splice Sites, pubmed-meshheading:11419941-Rats, pubmed-meshheading:11419941-Salivary Glands
pubmed:year
2001
pubmed:articleTitle
cDNA and genomic cloning of lacritin, a novel secretion enhancing factor from the human lacrimal gland.
pubmed:affiliation
Department of Cell Biology, University of Virginia, Charlottesville, VA 22908, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.