Linked Life Data

11418640

Source:http://linkedlifedata.com/resource/pubmed/id/11418640

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-6-21
pubmed:abstractText
CD44 is a widely expressed integral membrane glycoprotein that serves as a specific adhesion receptor for the extracellular matrix glycosaminoglycan hyaluronan. CD44 participates in a variety of physiological and pathological processes through its role in cell adhesion. Under appropriate conditions, the ectodomain of CD44 is proteolytically removed from the cell surface. In this study we show that excessive CD44 shedding can be induced in mouse fibroblasts and monocytes upon exposure of these cells to a CD44-specific Ab immobilized on plastic, whereas treatment with phorbol ester induces significantly enhanced CD44 release from the monocytes only. CD44 shedding proceeds normally in fibroblasts and monocytes deficient in TNF-alpha converting enzyme (TACE), a sheddase involved in the processing of several substrates. Conversely, activation of the CD44 protease has no effect on the release of TNF-alpha from TACE-expressing cells, although the same metalloprotease inhibitor effectively blocks both TACE and the CD44 sheddase. Concomitant with anti-CD44 Ab- or phorbol ester-induced CD44 shedding, dramatic changes are observed in cell morphology and the structure of the actin cytoskeleton. Disruption of actin assembly with cytochalasin reduces CD44 shedding, but not the release of TNF-alpha. Moreover, pharmacological activation of Rho family GTPases Rac1 and Cdc42, which regulate actin filament assembly into distinct cytoskeletal structures, has a profound effect on CD44 release. We conclude that the CD44 sheddase and TACE are distinct enzymes, and that Ab- and phorbol ester-enhanced cleavage of CD44 is controlled in a cell type-dependent fashion by Rho GTPases through the cytoskeleton.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
167
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
123-31
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11418640-ADAM Proteins, pubmed-meshheading:11418640-Animals, pubmed-meshheading:11418640-Antibodies, Monoclonal, pubmed-meshheading:11418640-Antigens, CD44, pubmed-meshheading:11418640-Bone Marrow Cells, pubmed-meshheading:11418640-Cell Adhesion, pubmed-meshheading:11418640-Cell Line, Transformed, pubmed-meshheading:11418640-Cell Membrane, pubmed-meshheading:11418640-Cells, Cultured, pubmed-meshheading:11418640-Culture Media, Conditioned, pubmed-meshheading:11418640-Cytoskeleton, pubmed-meshheading:11418640-Fibroblasts, pubmed-meshheading:11418640-Genes, myc, pubmed-meshheading:11418640-Genes, ras, pubmed-meshheading:11418640-Hydrolysis, pubmed-meshheading:11418640-Kinetics, pubmed-meshheading:11418640-Metalloendopeptidases, pubmed-meshheading:11418640-Mice, pubmed-meshheading:11418640-Mice, Inbred BALB C, pubmed-meshheading:11418640-Monocytes, pubmed-meshheading:11418640-RNA, Messenger, pubmed-meshheading:11418640-Synovial Membrane, pubmed-meshheading:11418640-Tetradecanoylphorbol Acetate
pubmed:year
2001
pubmed:articleTitle
Antibody-induced shedding of CD44 from adherent cells is linked to the assembly of the cytoskeleton.
pubmed:affiliation
Departments of. Biochemistry and Orthopedic Surgery, Section of Biochemistry and Molecular Biology, Rush University at Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL 60612, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.