Linked Life Data

11418472

Source:http://linkedlifedata.com/resource/pubmed/id/11418472

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-6-21
pubmed:abstractText
It was recently reported that autoreactive CD4(+) T cells to glycoprotein IIb-IIIa (GPIIb-IIIa) mediate antiplatelet autoantibody production in patients with immune thrombocytopenic purpura (ITP). To further examine the antigenic specificity of the GPIIb-IIIa-reactive T cells, 6 recombinant fragments encoding different portions of GPIIbalpha or GPIIIa were generated and tested for their ability to stimulate antigen-specific T-cell proliferation and anti-GPIIb-IIIa antibody production in vitro. T cells from the peripheral blood of 25 patients with ITP and 10 healthy donors proliferated in response to recombinant GPIIb-IIIa fragments in various combinations. The amino-terminal portions of both GPIIbalpha and GPIIIa (IIbalpha18-259 and IIIa22-262) were frequently recognized (60% and 64%, respectively) compared with other fragments (4%-28%) in patients with ITP, but this tendency was not detected in healthy donors. In subsequent analyses in patients with ITP, T-cell reactivities to IIbalpha18-259 and IIIa22-262 were consistently detected, whereas those to other fragments were sometimes lost. In vitro antigenic stimulation of peripheral blood mononuclear cells with IIbalpha18-259 or IIIa22-262 promoted the synthesis of anti-GPIIb-IIIa antibodies in patients with ITP, but not in healthy donors. Of 15 CD4(+) T-cell lines specific for platelet-derived GPIIb-IIIa generated from 5 patients with ITP, 13 lines recognized IIbalpha18-259, IIIa22-262, or both. T-cell lines reactive to IIbalpha18-259 or IIIa22-262 promoted the production of anti-GPIIb-IIIa antibodies that were capable of binding to normal platelet surfaces. These results indicate that the immunodominant epitopes recognized by pathogenic CD4(+) T cells in patients with ITP are located within the amino-terminal portions of both GPIIbalpha and GPIIIa.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
130-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11418472-Adult, pubmed-meshheading:11418472-Aged, pubmed-meshheading:11418472-Antibodies, pubmed-meshheading:11418472-CD4-Positive T-Lymphocytes, pubmed-meshheading:11418472-Case-Control Studies, pubmed-meshheading:11418472-Cell Culture Techniques, pubmed-meshheading:11418472-Epitope Mapping, pubmed-meshheading:11418472-Female, pubmed-meshheading:11418472-Humans, pubmed-meshheading:11418472-Immunodominant Epitopes, pubmed-meshheading:11418472-Lymphocyte Activation, pubmed-meshheading:11418472-Male, pubmed-meshheading:11418472-Middle Aged, pubmed-meshheading:11418472-Peptide Fragments, pubmed-meshheading:11418472-Platelet Glycoprotein GPIIb-IIIa Complex, pubmed-meshheading:11418472-Purpura, Thrombocytopenic, Idiopathic, pubmed-meshheading:11418472-Recombinant Fusion Proteins
pubmed:year
2001
pubmed:articleTitle
Immunodominant epitopes on glycoprotein IIb-IIIa recognized by autoreactive T cells in patients with immune thrombocytopenic purpura.
pubmed:affiliation
Institute for Advanced Medical Research and the Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan. kuwanam@sc.itc.keio.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't