Source:http://linkedlifedata.com/resource/pubmed/id/11418182
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2001-6-21
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pubmed:abstractText |
The murine Nd1 gene encodes a novel Kelch family protein and expresses two forms of mRNA, long (Nd1-L) and short (Nd1-S), in various tissues. We characterized the genomic organization of the Nd1 gene, and found that Nd1-L and Nd1-S consist of 16 and nine exons respectively, and that exons I-VIII are shared between them. Three transcription initiation sites were identified in the 5'-flanking region and the most 3' side (+1) is likely to be a major one. Promoter analysis revealed that the region between positions -247 and -86 was sufficient for expression, and that two Sp1-binding sites and one NF-kappaB-binding site in the region were critical for promoter activity. Furthermore, the promoter region lacks a TATA and a CAAT box and has a highly GC-rich region with two important Sp1-binding sites. These characteristics of the Nd1 gene promoter are similar to the properties of housekeeping genes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
1519
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
167-74
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11418182-Amino Acid Sequence,
pubmed-meshheading:11418182-Animals,
pubmed-meshheading:11418182-Base Sequence,
pubmed-meshheading:11418182-DNA,
pubmed-meshheading:11418182-Genes, Reporter,
pubmed-meshheading:11418182-Luciferases,
pubmed-meshheading:11418182-Mice,
pubmed-meshheading:11418182-Molecular Sequence Data,
pubmed-meshheading:11418182-Promoter Regions, Genetic,
pubmed-meshheading:11418182-Proteins,
pubmed-meshheading:11418182-Transcription, Genetic,
pubmed-meshheading:11418182-Tumor Cells, Cultured
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pubmed:year |
2001
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pubmed:articleTitle |
Nd1, a novel murine Kelch family protein, may play the role of a housekeeping gene.
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pubmed:affiliation |
Department of Developmental Genetics (H2), Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Japan.
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pubmed:publicationType |
Journal Article
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