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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2001-6-21
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pubmed:abstractText |
Chlorophyll (Chl) molecules attached to plant light-harvesting complexes (LHC) differ in their spectral behavior. While most Chl a and Chl b molecules give rise to absorption bands between 645 nm and 670 nm, some special Chls absorb at wavelengths longer than 700 nm. Among the Chl a/b-antennae of higher plants these are found exclusively in LHC I. In order to assign this special spectral property to one chlorophyll species we reconstituted LHC of both photosystem I (Lhca4) and photosystem II (Lhcb1) with carotenoids and only Chl a or Chl b and analyzed the effect on pigment binding, absorption and fluorescence properties. In both LHCs the Chl-binding sites of the omitted Chl species were occupied by the other species resulting in a constant total number of Chls in these complexes. 77-K spectroscopic measurements demonstrated that omission of Chl b in refolded Lhca4 resulted in a loss of long-wavelength absorption and 730-nm fluorescence emission. In Lhcb1 with only Chl b long-wavelength emission was preserved. These results clearly demonstrate the involvement of Chl b in establishing long-wavelength properties.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
499
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
27-31
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11418105-Absorption,
pubmed-meshheading:11418105-Binding Sites,
pubmed-meshheading:11418105-Centrifugation, Density Gradient,
pubmed-meshheading:11418105-Chlorophyll,
pubmed-meshheading:11418105-Light-Harvesting Protein Complexes,
pubmed-meshheading:11418105-Lycopersicon esculentum,
pubmed-meshheading:11418105-Photosynthetic Reaction Center Complex Proteins,
pubmed-meshheading:11418105-Photosystem I Protein Complex,
pubmed-meshheading:11418105-Photosystem II Protein Complex,
pubmed-meshheading:11418105-Pigments, Biological,
pubmed-meshheading:11418105-Plant Leaves,
pubmed-meshheading:11418105-Protein Binding,
pubmed-meshheading:11418105-Spectrometry, Fluorescence,
pubmed-meshheading:11418105-Spinacia oleracea,
pubmed-meshheading:11418105-Temperature
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pubmed:year |
2001
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pubmed:articleTitle |
Chlorophyll b is involved in long-wavelength spectral properties of light-harvesting complexes LHC I and LHC II.
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pubmed:affiliation |
Institut für Allgemeine Botanik, Johannes-Gutenberg Universität, Mainz, Germany. vschmid@mail.uni-mainz.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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