Linked Life Data

11418008

Source:http://linkedlifedata.com/resource/pubmed/id/11418008

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-6-21
pubmed:abstractText
This study describes an advantageous, effective protocol for detecting K-Ras mutations in human stool as a prototype screen for colorectal carcinoma (CRC), the third most common malignancy in the United States. A reliable screening test that detects early lesions would contribute to a decrease in mortality. Currently, the only noninvasive screen for CRC is the hemeoccult, test which has a high false-positive rate. Previously, several investigators have identified genetic biomarkers for CRC in stool DNA. The K-Ras oncogene, mutated in 46-50% of CRC tumors, serves as one molecular marker by which stool samples may be evaluated for early detection of adenocarcinomas. DNA was isolated from stool samples by a new method we specifically designed for extracting high-quality DNA using tetradecyltrimethylammonium oxalate [Catrimox-14, Iowa Biotechnology Corp., (currently Qiagen)]. This protocol produces an optimal yield of high-purity DNA, suitable for genotyping. Detection of the human gene in stool samples was enhanced by hybrid selection of the K-Ras sequences, polymerase chain reaction, and single-strand conformation polymorphism. Tumor tissue and preoperative stool samples for eight patients were K-Ras genotyped and compared; stool samples from two asymptomatic, healthy patients were also evaluated in a double-blind format. In seven of eight samples (87%), the genotypes of the stool and colon tissue DNA were the same. Both healthy patients showed wild-type K-Ras. This protocol shows promise for the development of an efficient and accurate screen for CRC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0014-4800
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Academic Press.
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
289-301
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11418008-Colorectal Neoplasms, pubmed-meshheading:11418008-DNA, pubmed-meshheading:11418008-DNA, Neoplasm, pubmed-meshheading:11418008-Deoxyribonuclease EcoRI, pubmed-meshheading:11418008-Feces, pubmed-meshheading:11418008-Genes, ras, pubmed-meshheading:11418008-Humans, pubmed-meshheading:11418008-Indicators and Reagents, pubmed-meshheading:11418008-Mutation, pubmed-meshheading:11418008-Polymerase Chain Reaction, pubmed-meshheading:11418008-Polymorphism, Single-Stranded Conformational, pubmed-meshheading:11418008-Quaternary Ammonium Compounds, pubmed-meshheading:11418008-Reference Values, pubmed-meshheading:11418008-Reproducibility of Results, pubmed-meshheading:11418008-Site-Specific DNA-Methyltransferase (Adenine-Specific), pubmed-meshheading:11418008-Trimethyl Ammonium Compounds, pubmed-meshheading:11418008-Tumor Markers, Biological
pubmed:year
2001
pubmed:articleTitle
Detection of the mutated K-Ras biomarker in colorectal carcinoma.
pubmed:affiliation
Department of Pathology, Yale University, School of Medicine, New Haven, Connecticut, USA.
pubmed:publicationType
Journal Article