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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 1
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pubmed:dateCreated |
2001-6-20
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pubmed:abstractText |
We report that exposure of mouse embryonic fibroblasts to transforming growth factor beta-1 (TGFbeta-1) (5 ng/ml) results in a strong activation of p8 mRNA expression that precedes the induction of cell growth. Involvement of the p8 promoter in the regulation was demonstrated by using a p8-chloramphenicol acetyltransferase construct. We therefore speculated that p8 might be a mediator of TGFbeta-1 in these cells. The incorporation of [(3)H]thymidine on treatment with TGFbeta-1 was indeed significantly higher in p8(+/+) fibroblasts than in p8(-/-) fibroblasts. Smad transcriptional activity was used as marker of the TGFbeta-1 signalling pathway, to probe the lower p8(-/-) response to TGFbeta-1. Two Smad-binding elements (SBEs)-luciferase constructs were transfected into p8(-/-) and p8(+/+) embryonic fibroblasts before treatment with TGFbeta-1. A lower level of Smad transactivation was observed in p8(-/-) embryonic fibroblasts, under basal conditions and after stimulation with TGFbeta-1. To test whether Smad underexpression in p8(-/-) cells was actually due to p8 depletion, p8(-/-) embryonic fibroblasts were transfected with a human p8 expression plasmid together with an SBE-luciferase construct. The expression of p8 restored Smad transactivation in unstimulated and TGFbeta-1-treated cells to the level found in p8(+/+) cells. We concluded that TGFbeta-1 activates p8 expression, which in turn enhances the Smad-transactivating function responsible for TGFbeta-1 activity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-10082546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-10092851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-10377453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-10403827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-10438498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-10493524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-10510303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-10519421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-10635333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-10660041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-10706706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-10775259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-11056169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-2171777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-2177343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-2208284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-2217201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-3169576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-7479086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-7545677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-7882352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-8103211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-8448037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-8843198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-9368066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-9393997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-9405444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-9490789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-9694870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-9759503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11415456-9797468
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Basic Helix-Loop-Helix...,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/High Mobility Group Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nupr1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/P8 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
357
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
249-53
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11415456-Animals,
pubmed-meshheading:11415456-Mice,
pubmed-meshheading:11415456-Embryo, Mammalian,
pubmed-meshheading:11415456-Luciferases,
pubmed-meshheading:11415456-DNA,
pubmed-meshheading:11415456-Fibroblasts,
pubmed-meshheading:11415456-Thymidine,
pubmed-meshheading:11415456-Growth Substances,
pubmed-meshheading:11415456-Cells, Cultured,
pubmed-meshheading:11415456-RNA, Messenger,
pubmed-meshheading:11415456-Neoplasm Proteins,
pubmed-meshheading:11415456-Homozygote,
pubmed-meshheading:11415456-Transcription, Genetic,
pubmed-meshheading:11415456-Mice, Inbred C57BL,
pubmed-meshheading:11415456-3T3 Cells,
pubmed-meshheading:11415456-Gene Expression Regulation,
pubmed-meshheading:11415456-DNA-Binding Proteins,
pubmed-meshheading:11415456-Transfection,
pubmed-meshheading:11415456-Transcriptional Activation,
pubmed-meshheading:11415456-High Mobility Group Proteins,
pubmed-meshheading:11415456-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:11415456-Gene Deletion,
pubmed-meshheading:11415456-Recombinant Fusion Proteins
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