Source:http://linkedlifedata.com/resource/pubmed/id/11413235
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2001-6-19
|
| pubmed:abstractText |
The coupling of opioid receptor-like (ORL1) receptors to adenylyl cyclase has been investigated in specific layers of the rat main olfactory bulb. Membranes prepared from the olfactory nerve-glomerular layer (ON-G layer), external plexiform layer (EP layer) and granule cell layer (GR layer) displayed specific binding sites for [(3)H]-nociceptin/orphanin FQ ([(3)H]Noc/OFQ). In each layer, the presence of high-and low-affinity binding sites, with K(D) values in the picomolar and nanomolar range, respectively, was detected. The binding of [(3)H]Noc/OFQ was displaced by unlabelled Noc/OFQ, but not by opioid antagonists. In each layer, Noc/OFQ significantly stimulated [(35)S]GTPgammaS binding with nanomolar potencies. In ON-G layer, Noc/OFQ inhibited basal adenylyl cyclase activity and the enzyme stimulations by corticotropin releasing hormone (CRH), Ca(2+)/calmodulin (Ca(2+)/CaM) and forskolin (FSK). In EP layer, Noc/OFQ inhibited Ca(2+)/CaM-and FSK-stimulated enzyme activities. Conversely, in GR layer the peptide stimulated basal cyclase activity and potentiated the enzyme activation by CRH. The Noc/OFQ stimulation was counteracted by the GDP-bound form of the alpha subunit of transducin and was mimicked by transducin betagamma subunits. In the same tissue layer, Ca(2+)/CaM-and FSK-stimulated enzyme activities were inhibited. Naloxone failed to antagonize all the actions of Noc/OFQ. Western blot and RT-PCR analysis revealed the expression of Ca(2+)-insensitive and -sensitive adenylyl cyclases in the three layers. These results demonstrate that in rat main olfactory bulb ORL1 receptors can differentially affect distinct forms of adenylyl cyclase in a layer specific manner.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calmodulin,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine 5'-O-(3-Thiotriphosphate),
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Opioid Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfur Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium,
http://linkedlifedata.com/resource/pubmed/chemical/Vasodilator Agents,
http://linkedlifedata.com/resource/pubmed/chemical/nociceptin,
http://linkedlifedata.com/resource/pubmed/chemical/nociceptin receptor
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0022-3042
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
77
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1520-30
|
| pubmed:dateRevised |
2005-12-20
|
| pubmed:meshHeading |
pubmed-meshheading:11413235-Adenylate Cyclase,
pubmed-meshheading:11413235-Animals,
pubmed-meshheading:11413235-Calcium,
pubmed-meshheading:11413235-Calmodulin,
pubmed-meshheading:11413235-Enzyme Activation,
pubmed-meshheading:11413235-Forskolin,
pubmed-meshheading:11413235-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:11413235-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:11413235-Isoenzymes,
pubmed-meshheading:11413235-Male,
pubmed-meshheading:11413235-Olfactory Bulb,
pubmed-meshheading:11413235-Opioid Peptides,
pubmed-meshheading:11413235-RNA, Messenger,
pubmed-meshheading:11413235-Radioligand Assay,
pubmed-meshheading:11413235-Rats,
pubmed-meshheading:11413235-Rats, Sprague-Dawley,
pubmed-meshheading:11413235-Receptors, Opioid,
pubmed-meshheading:11413235-Sulfur Radioisotopes,
pubmed-meshheading:11413235-Tritium,
pubmed-meshheading:11413235-Vasodilator Agents
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Dual coupling of opioid receptor-like (ORL1) receptors to adenylyl cyclase in the different layers of the rat main olfactory bulb.
|
| pubmed:affiliation |
Departments of Neuroscience and Medical Sciences, Section of Biochemical Pharmacology, University of Cagliari, Cagliari, Italy. onali@unica.it
|
| pubmed:publicationType |
Journal Article
|