Source:http://linkedlifedata.com/resource/pubmed/id/11410338
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2001-6-18
|
| pubmed:abstractText |
In order to investigate whether earthworm fibrinolytic enzyme III-1 (EFE-III-1) isolated from Lumbricus rubellus is capable of transporting into blood through intestinal epithelium and keeping its biological function in circulation, we have raised an antibody against EFE-III-1. The immunological results showed that 10-15% of intact EFE-III-1 was absorbed by the intestinal epithelium with the incubation chamber method [Vilhardt and Lundin, Acta Physiol. Scand. 126 (1986) 601-607]. The enzyme could be detected in the intestinal epithelial cells by immunohistochemistry. Furthermore, immunoreactive intact EFE-III-1 was found in serum or plasma after intraperitoneal injection of rats. Approx. 10% of the full-size enzyme could transport through the intestinal epithelium. The maximum remaining activity in blood could be assayed around 60 min after the intraperitoneal injection.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0006-3002
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
1526
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
286-92
|
| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11410338-Amino Acid Sequence,
pubmed-meshheading:11410338-Animals,
pubmed-meshheading:11410338-Biological Transport,
pubmed-meshheading:11410338-Cell Membrane Permeability,
pubmed-meshheading:11410338-Duodenum,
pubmed-meshheading:11410338-Endopeptidases,
pubmed-meshheading:11410338-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11410338-Epithelium,
pubmed-meshheading:11410338-Fibrinolytic Agents,
pubmed-meshheading:11410338-Frozen Sections,
pubmed-meshheading:11410338-Immunoblotting,
pubmed-meshheading:11410338-Immunohistochemistry,
pubmed-meshheading:11410338-Injections, Intraperitoneal,
pubmed-meshheading:11410338-Intestinal Absorption,
pubmed-meshheading:11410338-Molecular Sequence Data,
pubmed-meshheading:11410338-Oligochaeta,
pubmed-meshheading:11410338-Rats
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Some features of intestinal absorption of intact fibrinolytic enzyme III-1 from Lumbricus rubellus.
|
| pubmed:affiliation |
Laboratory of Visual Information Processing, Institute of Biophysics, Baiao Pharmaceuticals Beijing C.L., Chinese Academy of Sciences, 15 Da Tun Rd, Chaoyang District, 100101, Beijing, PR China.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|