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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2001-6-15
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pubmed:abstractText |
We have shown that liver myofibroblasts stimulate in vitro invasion of hepatocellular carcinoma cell lines through a hepatocyte growth factor/urokinase-dependent mechanism. Resveratrol, a grapevine-derived polyphenol, has been shown to inhibit cellular events associated with tumor initiation, promotion and progression. The aim of this study was to evaluate the effects of trans-resveratrol on invasion of the human hepatoma cell line HepG2. Cell invasion was assessed using a Boyden chamber assay. Activation of the HGF signal transduction pathways was evaluated by Western blot with phospho-specific antibodies. Urokinase expression was measured by RT-PCR and zymography. Trans-resveratrol decreased hepatocyte growth factor-induced cell scattering and invasion. It also decreased cell proliferation without evidence for cytotoxicity or apoptosis. Trans-resveratrol did not decrease the level of the hepatocyte growth factor receptor c-met and did not impede the hepatocyte growth factor-induced increase in c-met precursor synthesis. Moreover, trans-resveratrol did not decrease hepatocyte growth factor-induced c-met autophosphorylation, or Akt-1 or extracellular-regulated kinases-1 and -2 activation. Finally, it did not decrease urokinase expression and did not block the catalytic activity of urokinase. In conclusion, our results demonstrate that trans-resveratrol decreases hepatocyte growth factor-induced HepG2 cell invasion by an as yet unidentified post-receptor mechanism.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AKT1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents, Phytogenic,
http://linkedlifedata.com/resource/pubmed/chemical/Flavonoids,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase...,
http://linkedlifedata.com/resource/pubmed/chemical/PLAUR protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Phenols,
http://linkedlifedata.com/resource/pubmed/chemical/Poly(ADP-ribose) Polymerases,
http://linkedlifedata.com/resource/pubmed/chemical/Polymers,
http://linkedlifedata.com/resource/pubmed/chemical/Polyphenols,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-met,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Urokinase Plasminogen...,
http://linkedlifedata.com/resource/pubmed/chemical/Stilbenes,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazolium Salts,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Urokinase-Type Plasminogen Activator,
http://linkedlifedata.com/resource/pubmed/chemical/resveratrol,
http://linkedlifedata.com/resource/pubmed/chemical/thiazolyl blue
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1019-6439
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
83-8
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:11408926-Antineoplastic Agents, Phytogenic,
pubmed-meshheading:11408926-Carcinoma, Hepatocellular,
pubmed-meshheading:11408926-Cell Division,
pubmed-meshheading:11408926-Cell Survival,
pubmed-meshheading:11408926-Flavonoids,
pubmed-meshheading:11408926-Hepatocyte Growth Factor,
pubmed-meshheading:11408926-Humans,
pubmed-meshheading:11408926-Liver Neoplasms,
pubmed-meshheading:11408926-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:11408926-Neoplasm Invasiveness,
pubmed-meshheading:11408926-Phenols,
pubmed-meshheading:11408926-Phosphorylation,
pubmed-meshheading:11408926-Poly(ADP-ribose) Polymerases,
pubmed-meshheading:11408926-Polymers,
pubmed-meshheading:11408926-Polyphenols,
pubmed-meshheading:11408926-Protein-Serine-Threonine Kinases,
pubmed-meshheading:11408926-Proto-Oncogene Proteins,
pubmed-meshheading:11408926-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:11408926-Proto-Oncogene Proteins c-met,
pubmed-meshheading:11408926-RNA, Messenger,
pubmed-meshheading:11408926-Receptors, Cell Surface,
pubmed-meshheading:11408926-Receptors, Urokinase Plasminogen Activator,
pubmed-meshheading:11408926-Stilbenes,
pubmed-meshheading:11408926-Tetrazolium Salts,
pubmed-meshheading:11408926-Thiazoles,
pubmed-meshheading:11408926-Tumor Cells, Cultured,
pubmed-meshheading:11408926-Urokinase-Type Plasminogen Activator
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pubmed:year |
2001
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pubmed:articleTitle |
Trans-resveratrol, a grapevine-derived polyphenol, blocks hepatocyte growth factor-induced invasion of hepatocellular carcinoma cells.
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pubmed:affiliation |
Groupe de Recherches pour l'Etude du Foie, INSERM E9917, Universite Victor Segalen Bordeaux 2, 33076 Bordeaux cedex, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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