Linked Life Data

11408225

Source:http://linkedlifedata.com/resource/pubmed/id/11408225

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2001-6-15
pubmed:abstractText
A major limitation of the National Committee for Clinical Laboratory Standards M27-A methodology is reliable detection of amphotericin B (AMB) resistance. The results obtained by using Iso-Sensitest, a synthetic medium, to detect AMB resistance were analyzed and compared with those obtained with RPMI and antibiotic medium 3 (AM3). The ability to detect AMB resistance with RPMI is not enhanced by using a higher inoculum, glucose supplementation at a final concentration of 20 g/liter, spectrophotometric reading, or 24 h of incubation time. Testing using AM3 and an inoculum of 10(3) CFU/ml detects resistance. Identification of resistant isolates is not improved by glucose supplementation, changes in reading method, or changes in incubation time. However, the use of AM3 as assay medium and an inoculum of 10(5) CFU/ml did not allow detection of AMB resistance. Testing using Iso-Sensitest medium appears to be similar to AM3 in detecting resistance. The most pronounced discrimination is achieved by testing in Iso-Sensitest supplemented with glucose and spectrophotometric reading after 24 h of incubation. The reproducibility of MIC testing was greatest for Iso-Sensitest-based procedures. Use of Iso-Sensitest produces both highly reproducible MICs and reliable identification of AMB-resistant Candida isolates.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11408225-10980162, http://linkedlifedata.com/resource/pubmed/commentcorrection/11408225-11158101, http://linkedlifedata.com/resource/pubmed/commentcorrection/11408225-7559186, http://linkedlifedata.com/resource/pubmed/commentcorrection/11408225-7785993, http://linkedlifedata.com/resource/pubmed/commentcorrection/11408225-8062537, http://linkedlifedata.com/resource/pubmed/commentcorrection/11408225-8141578, http://linkedlifedata.com/resource/pubmed/commentcorrection/11408225-8585737, http://linkedlifedata.com/resource/pubmed/commentcorrection/11408225-8968923, http://linkedlifedata.com/resource/pubmed/commentcorrection/11408225-9114154, http://linkedlifedata.com/resource/pubmed/commentcorrection/11408225-9466531, http://linkedlifedata.com/resource/pubmed/commentcorrection/11408225-9681198, http://linkedlifedata.com/resource/pubmed/commentcorrection/11408225-9738026, http://linkedlifedata.com/resource/pubmed/commentcorrection/11408225-9884840
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0066-4804
pubmed:author
pubmed:issnType
Print
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2070-4
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Detection of resistance to amphotericin B in Candida isolates by using Iso-Sensitest broth.
pubmed:affiliation
Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Madrid, Spain. mcuenca-estrella@isciii.es
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't