Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2001-6-14
pubmed:abstractText
Cyclin D1, one of the G(1) cyclins, is frequently overexpressed in several types of carcinomas and is thought to play an important role in tumorigenesis and tumor progression including hepatocellular carcinoma. We constructed a retrovirus vector-carrying rat cyclin D1 cDNA in the reverse orientation, resulting in expression of antisense (AS) cyclin D1 mRNA. For efficient transduction of this recombinant retrovirus, two-step gene transfer was performed. The rat hepatoma cell line (dRLh84) was infected with this recombinant retrovirus after preinfection with adenovirus expressing the retrovirus receptor. In the rat hepatoma cells, AS cyclin D1 mRNA was expressed, inducing a decrease in the expression of endogenous cyclin D1 mRNA and an inhibition of cell growth. Moreover, two-step gene transfer of AS cyclin D1 into s.c. hepatoma xenografts resulted in inhibition of tumor growth and prolonged animal survival. In the virus-infected tumor xenografts, expression of cyclin D1 was immunohistochemically inhibited, and apoptosis of hepatoma cells was detected. These findings suggest that transduction of AS cyclin D1 is useful as an adjunct to standard treatments for hepatocellular carcinoma.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4779-83
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11406552-Animals, pubmed-meshheading:11406552-Blotting, Northern, pubmed-meshheading:11406552-Cell Division, pubmed-meshheading:11406552-Cyclin D1, pubmed-meshheading:11406552-DNA, Antisense, pubmed-meshheading:11406552-Gene Therapy, pubmed-meshheading:11406552-Gene Transfer Techniques, pubmed-meshheading:11406552-Humans, pubmed-meshheading:11406552-Immunohistochemistry, pubmed-meshheading:11406552-Liver Neoplasms, Experimental, pubmed-meshheading:11406552-Male, pubmed-meshheading:11406552-Mice, pubmed-meshheading:11406552-Mice, Inbred BALB C, pubmed-meshheading:11406552-Mice, Nude, pubmed-meshheading:11406552-RNA, Messenger, pubmed-meshheading:11406552-Rats, pubmed-meshheading:11406552-Rats, Wistar, pubmed-meshheading:11406552-Retroviridae, pubmed-meshheading:11406552-Transduction, Genetic, pubmed-meshheading:11406552-Tumor Cells, Cultured, pubmed-meshheading:11406552-Xenograft Model Antitumor Assays
pubmed:year
2001
pubmed:articleTitle
Transduction of antisense cyclin D1 using two-step gene transfer inhibits the growth of rat hepatoma cells.
pubmed:affiliation
Department of Internal Medicine II, Miyazaki Medical College, Kiyotake, Miyazaki 889-1692, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't