Linked Life Data

11406539

Source:http://linkedlifedata.com/resource/pubmed/id/11406539

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2001-6-14
pubmed:abstractText
We have generated a transgenic rat with the SV40 T antigen under probasin promoter control, allowing prostate-specific gene expression. Males demonstrate atypical epithelial cell proliferation in the prostate from 4 weeks of age and develop prostate carcinomas at 100% incidence before they are 15 weeks old. Castration at 5 weeks of age was found to inhibit the prostate tumor formation completely, whereas testosterone propionate administration induced marked cell proliferation as well as microinvasion in prostate carcinomas. Castration at 20 weeks of age, after tumor development, even with testosterone propionate treatment, induced complete tumor involution within 5 weeks. To investigate the underling processes, sequential histological changes were monitored 1, 2, 3, 7, 14, and 21 days after castration. At days 1-3, many apoptotic bodies and inflammatory cells, including foam cells, were observed, and clear glandular structures were no longer evident in the tumors. Seven days after castration, most glands were involved, and nuclei of the cells did not show atypia. After 14 and 21 days, only atrophic glands were observed. During this process, expression of caspase 3, caspase 6, BAX, bcl-x, TRPM-2, and MMP7 genes was apparently increased. Comparison of the gene expression profile between a prostate carcinoma in a transgenic animal and a normal prostate of a wild-type rat by a cDNA array technique was also conducted. The results suggested that our model is suitable to investigate mechanisms of carcinogenesis, including androgen dependence, involution, and apoptosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4693-700
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11406539-Androgen-Binding Protein, pubmed-meshheading:11406539-Androgens, pubmed-meshheading:11406539-Animals, pubmed-meshheading:11406539-Animals, Genetically Modified, pubmed-meshheading:11406539-Antigens, Polyomavirus Transforming, pubmed-meshheading:11406539-Female, pubmed-meshheading:11406539-Gene Expression Profiling, pubmed-meshheading:11406539-Gene Expression Regulation, Neoplastic, pubmed-meshheading:11406539-Male, pubmed-meshheading:11406539-Neoplasms, Hormone-Dependent, pubmed-meshheading:11406539-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:11406539-Orchiectomy, pubmed-meshheading:11406539-Promoter Regions, Genetic, pubmed-meshheading:11406539-Prostatic Neoplasms, pubmed-meshheading:11406539-Rats, pubmed-meshheading:11406539-Rats, Sprague-Dawley, pubmed-meshheading:11406539-Testosterone
pubmed:year
2001
pubmed:articleTitle
Prostate carcinomas developing in transgenic rats with SV40 T antigen expression under probasin promoter control are strictly androgen dependent.
pubmed:affiliation
First Department of Pathology, Nagoya City University Medical School, Nagoya 467-8601, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't