Source:http://linkedlifedata.com/resource/pubmed/id/11406418
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
2001-6-14
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| pubmed:abstractText |
To study how gamma-ray-induced germ-cell mutations are fixed at the early embryonic stage of the next generation, genomic alterations in the b locus mutants (colorless melanophores) detected during development in the medaka specific-locus test (SLT) were analyzed. First, nine anonymous DNA markers linked to the b locus were cloned and mapped into the region extending about 47cM surrounding the b locus. Next, losses of paternal alleles of these DNA markers were examined in each of the 51 gamma-ray-induced b locus mutants obtained after irradiation of sperm or spermatids. In these mutants, 47 were dominant lethals, three were semi-viable and one was viable. All the mutants examined had large deletions surrounding the b locus. One viable mutant had an interstitial deletion, while all the semi-viable and dominant lethal ones appeared to have terminal deletions. Deletions extending about 20-35cM were the most frequently observed in 18 of the 51 mutants examined. The largest one extended more than 40cM. These results suggest that most of the gamma-ray induced germ cell mutations recovered as total specific-locus mutants were accompanied by large genomic deletions, which eventually led the mutant embryos to dominant lethality.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
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| pubmed:issn |
0027-5107
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
458
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
19-29
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11406418-Animals,
pubmed-meshheading:11406418-Cell Line,
pubmed-meshheading:11406418-Centromere,
pubmed-meshheading:11406418-Chromosome Mapping,
pubmed-meshheading:11406418-Cloning, Molecular,
pubmed-meshheading:11406418-Embryo, Nonmammalian,
pubmed-meshheading:11406418-Female,
pubmed-meshheading:11406418-Gamma Rays,
pubmed-meshheading:11406418-Gene Deletion,
pubmed-meshheading:11406418-Genetic Markers,
pubmed-meshheading:11406418-Germ-Line Mutation,
pubmed-meshheading:11406418-Male,
pubmed-meshheading:11406418-Melanophores,
pubmed-meshheading:11406418-Oryzias,
pubmed-meshheading:11406418-Phenotype,
pubmed-meshheading:11406418-Polymerase Chain Reaction,
pubmed-meshheading:11406418-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:11406418-Spermatocytes
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Genomic analysis of gamma-ray-induced germ-cell mutations at the b locus recovered from the medaka specific-locus test.
|
| pubmed:affiliation |
Laboratory of Radiation Biology, Department of Biological Sciences, School of Science, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, 113-0033, Tokyo, Japan. fukasho@biol.s.u-tokyo.ac.jp
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|