|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1-2
|
|
pubmed:dateCreated |
2001-6-14
|
|
pubmed:abstractText |
The first version of the Human Combinatorial Antibody Library (HuCAL) is a single-chain Fv-based phage display library (HuCAL-scFv) with 2x10(9) members optimised for high-throughput generation and targeted engineering of human antibodies. 61% of the library genes code for functional scFv as judged by sequencing. We show here that since HuCAL-scFv antibodies are expressed in high levels in Escherichia coli, automated panning and screening in miniaturised settings (96- and 384-well format) have now become feasible. Additionally, the unique modular design of HuCAL-genes and -vectors allows the distinctly facilitated conversion of scFv into Fab, miniantibody and immunoglobulin formats, and the fusion with a variety of effector functions and tags not only convenient for therapeutic applications but also for high-throughput purification and detection. Thus, the HuCAL principle enables the rapid and high-throughput development of human antibodies by optimisation strategies proven useful in classical low molecular weight drug development. We demonstrate in this report that HuCAL is a very convenient source of human antibodies for various applications.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-C*06 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-C Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Fab Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage-1 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Library,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, erbB-2,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/immunoglobulin Fv,
http://linkedlifedata.com/resource/pubmed/chemical/tumor-associated antigen GA733
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0022-1759
|
|
pubmed:author |
pubmed-author:CamSS,
pubmed-author:DreierTT,
pubmed-author:FischerDD,
pubmed-author:HöslHH,
pubmed-author:KatiW MWM,
pubmed-author:KnappikAA,
pubmed-author:KrebsBB,
pubmed-author:KretzschmarTT,
pubmed-author:MargetMM,
pubmed-author:MengX QXQ,
pubmed-author:PackPP,
pubmed-author:RauchenbergerRR,
pubmed-author:ReiffertSS,
pubmed-author:RotheCC,
pubmed-author:SöhlemannPP,
pubmed-author:SchierRR,
pubmed-author:TesarMM,
pubmed-author:ThomassenEE,
pubmed-author:WölleJJ,
pubmed-author:WinterJJ
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
254
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
67-84
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:11406154-Animals,
pubmed-meshheading:11406154-Antibody Affinity,
pubmed-meshheading:11406154-Antibody Formation,
pubmed-meshheading:11406154-Antigens, Neoplasm,
pubmed-meshheading:11406154-Automation,
pubmed-meshheading:11406154-Blotting, Western,
pubmed-meshheading:11406154-CHO Cells,
pubmed-meshheading:11406154-Cell Adhesion Molecules,
pubmed-meshheading:11406154-Cloning, Molecular,
pubmed-meshheading:11406154-Cricetinae,
pubmed-meshheading:11406154-Flow Cytometry,
pubmed-meshheading:11406154-HL-60 Cells,
pubmed-meshheading:11406154-HLA-C Antigens,
pubmed-meshheading:11406154-HT29 Cells,
pubmed-meshheading:11406154-Humans,
pubmed-meshheading:11406154-Immunoglobulin Fab Fragments,
pubmed-meshheading:11406154-Immunoglobulin Fragments,
pubmed-meshheading:11406154-Immunoglobulin Variable Region,
pubmed-meshheading:11406154-Immunohistochemistry,
pubmed-meshheading:11406154-Intercellular Adhesion Molecule-1,
pubmed-meshheading:11406154-Macrophage-1 Antigen,
pubmed-meshheading:11406154-Peptide Library,
pubmed-meshheading:11406154-Precipitin Tests,
pubmed-meshheading:11406154-Receptor, Epidermal Growth Factor,
pubmed-meshheading:11406154-Receptor, erbB-2,
pubmed-meshheading:11406154-Recombinant Proteins,
pubmed-meshheading:11406154-Surface Plasmon Resonance
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
High-throughput generation and engineering of recombinant human antibodies.
|
|
pubmed:affiliation |
MorphoSys AG, Lena-Christ-Strasse 48, 82152 Martinsried, Germany.
|
|
pubmed:publicationType |
Journal Article
|
