Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-6-14
pubmed:abstractText
Mouse urothelium is disrupted just before birth, followed by a postnatal restoration process which includes cell proliferation, death and differentiation. We assessed urothelial proliferation by the expression of proliferating cell nuclear antigen (PCNA), desquamation by electron microscopy, and apoptosis by TUNEL staining and urothelial differentiation by the expression of uroplakins and cytokeratin 20 (CK20) as well as the apical plasma membrane maturation. Our results indicated that urothelial proliferation was high from birth until about the 14th postnatal day. A majority of basal cells and even occasional superficial cells were PCNA positive during the first 5 postnatal days. Cell death occurred during the first 9 postnatal days. Between birth and day 5, single cells underwent apoptosis, whereas between days 6 and 9 cells mainly desquamated. CK20 and uroplakins were expressed in all superficial cells in postnatal urothelium. Their subcellular distribution characteristically changed in accordance with the progressive differentiation of superficial cells. During the urothelial postnatal development, proliferation activity slowly decreases to the proliferatively quiescent urothelium of the adult animal. Apoptosis is present in the first 9 postnatal days and within a few days of this period it appears simultaneously with desquamation. Superficial urothelial cells gradually differentiate, which is reflected in the changeable morphology of the apical plasma membrane.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Intermediate Filament Proteins, http://linkedlifedata.com/resource/pubmed/chemical/KRT20 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Keratin-20, http://linkedlifedata.com/resource/pubmed/chemical/Krt20 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Tetraspanins, http://linkedlifedata.com/resource/pubmed/chemical/UPK1B protein, human, http://linkedlifedata.com/resource/pubmed/chemical/UPK2 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Upk1a protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Upk1b protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Upk2 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Uroplakin II, http://linkedlifedata.com/resource/pubmed/chemical/Uroplakin III, http://linkedlifedata.com/resource/pubmed/chemical/Uroplakin Ib
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0948-6143
pubmed:author
pubmed:issnType
Print
pubmed:volume
115
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
309-16
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:11405059-Animals, pubmed-meshheading:11405059-Apoptosis, pubmed-meshheading:11405059-Cell Division, pubmed-meshheading:11405059-Cell Membrane, pubmed-meshheading:11405059-Immunohistochemistry, pubmed-meshheading:11405059-In Situ Nick-End Labeling, pubmed-meshheading:11405059-Intermediate Filament Proteins, pubmed-meshheading:11405059-Keratin-20, pubmed-meshheading:11405059-Membrane Glycoproteins, pubmed-meshheading:11405059-Membrane Proteins, pubmed-meshheading:11405059-Mice, pubmed-meshheading:11405059-Microscopy, Confocal, pubmed-meshheading:11405059-Tetraspanins, pubmed-meshheading:11405059-Urinary Bladder, pubmed-meshheading:11405059-Uroplakin II, pubmed-meshheading:11405059-Uroplakin III, pubmed-meshheading:11405059-Uroplakin Ib, pubmed-meshheading:11405059-Urothelium
pubmed:year
2001
pubmed:articleTitle
Postnatal restoration of the mouse urinary bladder urothelium.
pubmed:affiliation
Institute of Cell Biology, Medical Faculty, Lipiceva 2, 1000 Ljubljana, Slovenia. aerman@ibmi.mf.uni-lj.si
pubmed:publicationType
Journal Article