Source:http://linkedlifedata.com/resource/pubmed/id/11403389
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2001-6-13
|
| pubmed:abstractText |
An improved subtraction hybridization technique was developed and evaluated. The hybridization is performed in a microplate with the subtractor-DNA immobilized in the plate while the probe-DNA is in solution. After hybridization the probe-specific DNA can easily be removed from the microwell and submitted to further analysis. This new technique has been successfully applied to generate several strain-specific PCR-primers for Lactococcus lactis subsp. lactis, Pediococcus spec., Saccharomyces spec. and Listeria monocytogenes.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0723-2020
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
24
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
108-15
|
| pubmed:meshHeading |
pubmed-meshheading:11403389-DNA Primers,
pubmed-meshheading:11403389-Food Microbiology,
pubmed-meshheading:11403389-Lactococcus lactis,
pubmed-meshheading:11403389-Listeria monocytogenes,
pubmed-meshheading:11403389-Nucleic Acid Hybridization,
pubmed-meshheading:11403389-Pediococcus,
pubmed-meshheading:11403389-Polymerase Chain Reaction,
pubmed-meshheading:11403389-Saccharomyces cerevisiae,
pubmed-meshheading:11403389-Species Specificity
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Subtraction hybridization in microplates: an improved method to generate strain-specific PCR primers.
|
| pubmed:affiliation |
Lehrstuhl für Mikrobiologie, Technische Universität München-Weihenstephan, Freising, Germany.
|
| pubmed:publicationType |
Journal Article
|