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rdf:type |
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lifeskim:mentions |
umls-concept:C0005953,
umls-concept:C0017262,
umls-concept:C0018183,
umls-concept:C0023810,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0125090,
umls-concept:C0185117,
umls-concept:C1367477,
umls-concept:C1611645,
umls-concept:C2911684
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pubmed:issue |
7
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pubmed:dateCreated |
2001-6-12
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pubmed:abstractText |
We established in previous studies that a constitutive lipopolysaccharide (LPS) receptor of low affinity is present on mouse bone marrow granulocytes (BMG). This yet-unidentified receptor is involved in the LPS-induced expression of a second LPS receptor, CD14. Because it has been claimed that L-selectin (CD62L) is a low-affinity LPS receptor in mature granulocytes (polymorphonuclear leukocytes), it may be asked whether this molecule could be the constitutive LPS receptor in BMG. We show in this study that L-selectin is constitutively present on BMG and is down-regulated after exposure of the cells to LPS. A phorbol ester induced a down-regulation of CD62L and blocked the LPS-induced expression of CD14. However, a metalloproteinase inhibitor (BB-3103) blocked the former but not the latter effect of PMA. We also observed an absence of cross-reactivity between LPS and a CD62L ligand (fucoidan) in binding studies with radiolabeled derivatives of the two agents. Furthermore, BMG from L-selectin-deficient mice expressed normal levels of CD14 in response to LPS. Taken together, these results demonstrate that in BMG, L-selectin is not the constitutive LPS receptor required for the LPS-induced expression of CD14.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-10359581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-10377245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-10500197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-10549626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-10681462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-10683379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-10708745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-10725698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-10733103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-11108799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-11123339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-2551036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-2827173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-3099289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-3814822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-6746643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-6866086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-7508438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-7518434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-7539045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-7541041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-7683322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-7690813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-8757614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-8973571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-9119455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-9198128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-9218616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-9301528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-9363686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-9380033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11401965-9541593
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD14,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/L-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Maleimides,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/bisindolylmaleimide I
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
69
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4287-94
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11401965-Animals,
pubmed-meshheading:11401965-Antigens, CD14,
pubmed-meshheading:11401965-Bone Marrow Cells,
pubmed-meshheading:11401965-Cells, Cultured,
pubmed-meshheading:11401965-Down-Regulation,
pubmed-meshheading:11401965-Enzyme Inhibitors,
pubmed-meshheading:11401965-Granulocytes,
pubmed-meshheading:11401965-Indoles,
pubmed-meshheading:11401965-L-Selectin,
pubmed-meshheading:11401965-Ligands,
pubmed-meshheading:11401965-Lipopolysaccharides,
pubmed-meshheading:11401965-Maleimides,
pubmed-meshheading:11401965-Mice,
pubmed-meshheading:11401965-Mice, Inbred C3H,
pubmed-meshheading:11401965-Mice, Inbred C57BL,
pubmed-meshheading:11401965-Mice, Knockout,
pubmed-meshheading:11401965-Protein Binding,
pubmed-meshheading:11401965-Protein Kinase C,
pubmed-meshheading:11401965-Tetradecanoylphorbol Acetate
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pubmed:year |
2001
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pubmed:articleTitle |
Down-modulation of L-selectin by lipopolysaccharide is not required for lipopolysaccharide-induced expression of CD14 in mouse bone marrow granulocytes.
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pubmed:affiliation |
Molecular Immunophysiology Unit, URA-1961 of the National Center for Scientific Research, Pasteur Institute, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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