Source:http://linkedlifedata.com/resource/pubmed/id/11399897
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2001-6-11
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| pubmed:abstractText |
In the kidney, epoxyeicosatrienoic acids (EETs) have been suggested to be endothelium-derived hyperpolarizing factors (EDHFs). The aim of the present study was to determine the contribution of EETs to the preglomerular vasodilation elicited by bradykinin. Sprague-Dawley rats were studied utilizing an in vitro perfused juxtamedullary nephron preparation. The afferent arteriolar diameter was determined and the diameter averaged 19 +/- 1 microm (n = 26) at a renal perfusion pressure of 100 mm Hg. Addition of 1, 10 and 100 nM bradykinin to the perfusate dose-dependently increased afferent arteriolar diameter by 5 +/- 1, 12 +/- 2 and 17 +/- 2%, respectively. The nitric oxide inhibitor N(omega)-nitro-L-arginine reduced bradykinin-induced afferent arteriolar vasodilation by 50%, and the diameter increased by 9 +/- 2% in response to 100 nM bradykinin. Epoxygenase inhibitors N-methylsulphonyl-6-(2-propargyloxyphenyl)hexanamide or miconazole greatly attenuated the nitric oxide-independent component of the vasodilation elicited by bradykinin. Cyclooxygenase (COX) inhibition attenuated the nitric oxide-independent vasodilation elicited by 1 nM bradykinin but did not significantly affect the vascular response to 100 nM bradykinin. Combined inhibition of nitric oxide, COX and epoxygenase pathways completely abolished bradykinin-mediated afferent arteriolar vasodilation. In additional studies, renal microvessels were isolated and incubated with bradykinin and samples were analyzed by NICI/GC/MS. Under control conditions, renal microvascular EET levels averaged 49 +/- 9 pg/mg/20 min (n = 7). In the presence of bradykinin, EET levels were significantly higher and averaged 81 +/- 11 pg/mg/20 min (n = 7). These data support the concept that EETs are EDHFs and contribute to the nitric oxide-independent afferent arteriolar vasodilation elicited by bradykinin.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/8,11,14-Eicosatrienoic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Amides,
http://linkedlifedata.com/resource/pubmed/chemical/Bradykinin,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Indomethacin,
http://linkedlifedata.com/resource/pubmed/chemical/N-methylsulfonyl-6-(2-propargyloxyph...,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitroarginine,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygenases,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/arachidonate epoxygenase
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| pubmed:status |
MEDLINE
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| pubmed:issn |
1018-1172
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2001 S. Karger AG, Basel
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| pubmed:issnType |
Print
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| pubmed:volume |
38
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
247-55
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11399897-8,11,14-Eicosatrienoic Acid,
pubmed-meshheading:11399897-Amides,
pubmed-meshheading:11399897-Animals,
pubmed-meshheading:11399897-Arterioles,
pubmed-meshheading:11399897-Bradykinin,
pubmed-meshheading:11399897-Culture Techniques,
pubmed-meshheading:11399897-Cyclooxygenase Inhibitors,
pubmed-meshheading:11399897-Cytochrome P-450 Enzyme System,
pubmed-meshheading:11399897-Enzyme Inhibitors,
pubmed-meshheading:11399897-Indomethacin,
pubmed-meshheading:11399897-Kidney,
pubmed-meshheading:11399897-Kinetics,
pubmed-meshheading:11399897-Male,
pubmed-meshheading:11399897-Microcirculation,
pubmed-meshheading:11399897-Nitric Oxide,
pubmed-meshheading:11399897-Nitroarginine,
pubmed-meshheading:11399897-Oxygenases,
pubmed-meshheading:11399897-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:11399897-Rats,
pubmed-meshheading:11399897-Rats, Sprague-Dawley,
pubmed-meshheading:11399897-Renal Circulation,
pubmed-meshheading:11399897-Vasodilation
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| pubmed:articleTitle |
Epoxygenase metabolites contribute to nitric oxide-independent afferent arteriolar vasodilation in response to bradykinin.
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| pubmed:affiliation |
Department of Physiology, Tulane University School of Medicine, New Orleans, La., USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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