Linked Life Data

11399059

Source:http://linkedlifedata.com/resource/pubmed/id/11399059

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-6-11
pubmed:abstractText
The interaction of stem cell factor (SCF) and c-kit is considered to be an important signaling event for the homeostasis of the epithelial barrier function in the intestinal tract. This study was designed to investigate the role of the SCF and c-kit signaling pathway in adhesion of intestinal epithelial cells (IECs) to fibronectin (FN) using primary cells. Fetal murine IECs were prepared from the small intestine of mouse fetus. The mRNAs coding for SCF in mesenchymes and c-kit in IECs were detected by reverse transcription-PCR. The expression of FN receptor VLA-5 on IECs was examined by flow cytometry. A cell adhesion assay showed that the stimulation of IECs with SCF increased the number of cells adhering to FN. Experiments using specific antibody against SCF indicated that this increase in cell adhesion was SCF-dependent. On the other hand, SCF did not influence the expression of VLA-5 on IECs. The IEC adhesion to FN was inhibited by specific antibody against the FN receptor (VLA-5), as well as competitive Arg-Gly-Asp (RGD) peptide. When alteration of intracellular signal transduction induced by SCF was examined, it was found that SCF stimulated a tyrosine-specific c-kit autophosphorylation cascade of IECs. Further, preincubation of IECs with an optimal concentration of genistein resulted in the inhibition of SCF-induced c-kit phosphorylation and adhesion of IECs to FN. These results suggested that adhesion of immature IECs to FN is regulated by activation of RGD-dependent VLA-5 through the SCF and c-kit signal transduction pathway. SCF, which may be produced by mesenchymes locally, is an important regulatory factor for the adhesion of immature IECs to basement membrane matrix via VLA-5 and FN interaction. This cytokine-regulated interaction between VLA-5 and FN may play an important role in the development and wound repair of the intestinal tract.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0014-4827
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Academic Press.
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
266
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
311-22
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11399059-Animals, pubmed-meshheading:11399059-Basement Membrane, pubmed-meshheading:11399059-Cell Adhesion, pubmed-meshheading:11399059-Cells, Cultured, pubmed-meshheading:11399059-Cytoskeletal Proteins, pubmed-meshheading:11399059-Epithelial Cells, pubmed-meshheading:11399059-Extracellular Matrix Proteins, pubmed-meshheading:11399059-Fetus, pubmed-meshheading:11399059-Fibronectins, pubmed-meshheading:11399059-Integrins, pubmed-meshheading:11399059-Intestinal Mucosa, pubmed-meshheading:11399059-Kinetics, pubmed-meshheading:11399059-Mice, pubmed-meshheading:11399059-Mice, Inbred C3H, pubmed-meshheading:11399059-Proto-Oncogene Proteins c-kit, pubmed-meshheading:11399059-RNA, Messenger, pubmed-meshheading:11399059-Receptors, Fibronectin, pubmed-meshheading:11399059-Signal Transduction, pubmed-meshheading:11399059-Stem Cell Factor
pubmed:year
2001
pubmed:articleTitle
Role of stem cell factor and c-kit signaling in regulation of fetal intestinal epithelial cell adhesion to fibronectin.
pubmed:affiliation
Department of Mucosal Immunology, Osaka University, Suita, Osaka, 565-0871, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't