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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
32
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pubmed:dateCreated |
2001-8-6
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pubmed:abstractText |
An early step in the process of HIV-1 entry into target cells is the activation of its envelope glycoprotein (GP120-GP41) to a fusogenic state upon binding to target cell CD4 and cognate co-receptor. Incubation of human immunodeficiency virus (HIV)-1 Env-expressing cells with an excess of CD4 and co-recepeptor-bearing target cells resulted in an influx of an impermeant nucleic acid-staining fluorescent dye into the Env-expressing cells. The dye influx occurred concomitant with cell fusion. No influx of dye into target cells was observed if they were incubated with an excess of Env-expressing cells. The permeabilization of Env-expressing cells was also triggered by CD4.co-receptor complexes attached to Protein G-Sepharose beads in the absence of target cells. The CD4 and co-receptor-induced permeabilization of Env-expressing cells occurred with the same specificity with respect to co-receptor usage as cell fusion. Natural ligands for the co-receptors and C-terminal GP41 peptide inhibitors of HIV-1 fusion blocked this effect. Our results indicate that the process of HIV-1 Env-mediated fusion is initiated by the destabilization of HIV-1 Env-expressing membranes. Further elucidation of these early intermediates may help identify and develop potential inhibitors of HIV-1 entry into cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Coloring Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Fluoresceins,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Envelope Protein gp120,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Envelope Protein gp41,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Organic Chemicals,
http://linkedlifedata.com/resource/pubmed/chemical/SYTOX Orange dye,
http://linkedlifedata.com/resource/pubmed/chemical/Sepharose,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/fluorexon
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
30335-41
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:11397808-3T3 Cells,
pubmed-meshheading:11397808-Animals,
pubmed-meshheading:11397808-Antigens, CD4,
pubmed-meshheading:11397808-Cell Line,
pubmed-meshheading:11397808-Cell Membrane,
pubmed-meshheading:11397808-Cells, Cultured,
pubmed-meshheading:11397808-Coloring Agents,
pubmed-meshheading:11397808-Fluoresceins,
pubmed-meshheading:11397808-Fluorescent Dyes,
pubmed-meshheading:11397808-HIV Envelope Protein gp120,
pubmed-meshheading:11397808-HIV Envelope Protein gp41,
pubmed-meshheading:11397808-HIV-1,
pubmed-meshheading:11397808-HeLa Cells,
pubmed-meshheading:11397808-Humans,
pubmed-meshheading:11397808-Kinetics,
pubmed-meshheading:11397808-Ligands,
pubmed-meshheading:11397808-Mice,
pubmed-meshheading:11397808-Organic Chemicals,
pubmed-meshheading:11397808-Precipitin Tests,
pubmed-meshheading:11397808-Protein Binding,
pubmed-meshheading:11397808-Sepharose,
pubmed-meshheading:11397808-Time Factors,
pubmed-meshheading:11397808-Vaccinia virus,
pubmed-meshheading:11397808-Viral Fusion Proteins
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pubmed:year |
2001
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pubmed:articleTitle |
Early intermediates in HIV-1 envelope glycoprotein-mediated fusion triggered by CD4 and co-receptor complexes.
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pubmed:affiliation |
Laboratory of Experimental and Computational Biology, Center for Cancer Research, NCI, National Institutes of Health, Frederick, Maryland 21702, USA.
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pubmed:publicationType |
Journal Article
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