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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2001-6-8
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pubmed:databankReference |
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pubmed:abstractText |
The functions of AML1 in hematopoietic differentiation are repressed by AML1-mutants including the AML1/ETO chimeric protein, which is seen in t(8;21) acute myeloid leukemia. Erythroid progenitors of the patients with t(8;21) AML expressed AML1/ETO. To investigate the effect of AML1/ETO in erythroid cells, we made a tetracycline-regulated AML1/ETO overexpression system in mouse erythroleukemic (MEL) cells. Enforced AML1/ETO repressed the terminal erythroid differentiation. Furthermore, we performed representational difference analysis using this MEL cell system to clone the downstream targets of AML1 in erythroid cell differentiation. We cloned a novel transmembrane protein, Art-1 (AML1-regulated transmembrane protein 1), which is a member of tetramembrane spanning superfamily. Art-1 expression was restricted in hematopoietic cells. It was upregulated by AML1 and downregulated by AML1/ETO in both erythroid and myeloid cells, and increased during erythroid cell differentiation. Art-1 may play an important role in the differentiation of erythroid cells, possibly as a direct downstream target of AML1.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AML1-ETO fusion protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Core Binding Factor Alpha 2 Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxamic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins, Fusion,
http://linkedlifedata.com/resource/pubmed/chemical/Phemx protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Runx1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tetracycline,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/trichostatin A
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
284
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
714-22
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:11396961-Amino Acid Sequence,
pubmed-meshheading:11396961-Animals,
pubmed-meshheading:11396961-Cell Differentiation,
pubmed-meshheading:11396961-Cloning, Molecular,
pubmed-meshheading:11396961-Core Binding Factor Alpha 2 Subunit,
pubmed-meshheading:11396961-DNA-Binding Proteins,
pubmed-meshheading:11396961-Enzyme Inhibitors,
pubmed-meshheading:11396961-Erythrocytes,
pubmed-meshheading:11396961-Hydroxamic Acids,
pubmed-meshheading:11396961-Leukemia, Erythroblastic, Acute,
pubmed-meshheading:11396961-Membrane Proteins,
pubmed-meshheading:11396961-Mice,
pubmed-meshheading:11396961-Molecular Sequence Data,
pubmed-meshheading:11396961-Myeloid Cells,
pubmed-meshheading:11396961-Oncogene Proteins, Fusion,
pubmed-meshheading:11396961-Proto-Oncogene Proteins,
pubmed-meshheading:11396961-RNA, Messenger,
pubmed-meshheading:11396961-Tetracycline,
pubmed-meshheading:11396961-Tissue Distribution,
pubmed-meshheading:11396961-Transcription Factors,
pubmed-meshheading:11396961-Tumor Cells, Cultured
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pubmed:year |
2001
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pubmed:articleTitle |
A hematopoietic-specific transmembrane protein, Art-1, is possibly regulated by AML1.
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pubmed:affiliation |
Department of Hematology/Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
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pubmed:publicationType |
Journal Article
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